利拉鲁肽对匹罗卡品致痫大鼠海马各区神经元凋亡影响.docVIP

利拉鲁肽对匹罗卡品致痫大鼠海马各区神经元凋亡影响 　　[摘要] 目的 探?利拉鲁肽对大鼠癫痫持续状态后海马各区神经元凋亡的影响。 方法 将雄性SD大鼠（n=54）随机分为空白对照组（n=6）、匹罗卡品模型组（SE组，n=24）、利拉鲁肽干预组（Liraglutide组，n=24）；并根据发作终止后的时间点（12 h，1 d，3 d，7 d）将SE组和Liraglutide组各分为4个亚组（n=6）。采用免疫组化技术检测海马CA3和DG区BCL2和BAX蛋白的表达。 结果 与SE组相比，Liraglutide组在SE后12 h、1 d、3 d时CA3区BCL2表达水平升高（P0.05）；在DG区，Liraglutide组在SE后1 d BCL2表达升高（P0.05）。Liraglutide组相较SE组，在SE后12 h，DG区BAX表达开始明显降低（P0.01）；在SE后1 d，CA3区BAX表达降低（P0.01）；且在SE后3 d，Liraglutide组DG区和CA3区BAX表达都高于空白对照组（P0.05）。 结论 利拉鲁肽通过抑制癫痫持续状态后海马CA3区和DG区神经元凋亡发挥神经保护作用。 　　[关键词] GLP-1；癫痫；海马；凋亡 　　[中图分类号] R742.1 [文献标识码] A [文章编号] 1673-9701（2018）10-0032-05 　　Effects of liraglutide on neuronal apoptosis in each zone of hippocampus in rats with epilepsy induced by pilocarpine 　　WANG Ruifang1 XUE Guofang2 LIAN Xia2 GAO Huizhong2 CAO Lijun2 ZHENG Jiying2 　　1.Shanxi Medical University，Taiyuan 030000，China；2.Department of Neurology，Shanxi Medical University Second Hospital，Taiyuan 030000，China 　　[Abstract] Objective To investigate the effect of liraglutide on neuronal apoptosis in each zone of hippocampus after persistent state of epilepsy in rats. Methods Male SD rats（n=54） were randomly divided into blank control group（n=6）， pilocarpine model group （SE group， n=24）， and liraglutide intervention group（Liraglutide group， n=24）； the SE group and liraglutide group were divided into 4 subgroups（n=6） according to the time point after the termination of attack（12 h， 1 d， 3 d， 7 d）. Immunohistochemistry was used to detect the expression of BCL2 and BAX proteins in CA3 and DG zones of hippocampus. Results In the liraglutide group， compared with the SE group， the expression of BCL2 in CA3 zone was increased at 12 hours， 1 day and 3 days after SE（P0.05）；in the DG zone， BCL2 expression was increased in liraglutide group one day after SE（P0.05）. In the liraglutide group， the expression of BAX was significantly decreased in DG zone at 12 hours after SE compared with SE group（P 　　[Key words] GLP-1；Epilepsy；Hippocampus；Apoptosis 　　癫痫持续状态（status epil