回阳生肌膏及其拆方对糖尿病慢性皮肤溃疡大鼠 血清白细胞介素1血栓素B2含量影响.docVIP

回阳生肌膏及其拆方对糖尿病慢性皮肤溃疡大鼠 血清白细胞介素1血栓素B2含量影响 　　摘要：目的观察回阳生肌膏及其拆方对糖尿病慢性皮肤溃疡大鼠白细胞介素-1α(IL-1α)和血栓素B2 (TXB2)含量的影响,探讨其促进糖尿病慢性皮肤溃疡愈合的作用机制。方法160只大鼠随机抽取6只作为空白组,不做任何处理,其余大鼠制备糖尿病模型,2周后随机分为1、3、5、7、14 d处理组,各处理组再分为正常组、模型组、回阳生肌膏组(全方组)、温阳益气拆方组(益气组)、活血生肌拆方组(活血组)。正常组及模型组给予凡士林纱条,全方组、益气组及活血组给予相应药物纱条。正常组采用皮肤缺损法,其他组采用复合因素制备慢性皮肤溃疡模型。在相应给药时间点处死各组大鼠,酶联免疫法检测各时点大鼠血清中IL-1α和TXB2的含量。结果IL-1α含量：给药3 d益气组显著高于空白组、模型组、全方组和活血组(P＜0.05)；给药5 d,全方组显著高于空白组和模型组(P＜0.05)；给药7 d,各给药组显著高于空白组和模型组(P＜0.05),全方组高于益气组、活血组(P＜0.05)；给药14 d,模型组和活血组低于空白组(P＜0.05)。TXB2含量：给药3 d,正常组和全方组高于空白组(P＜0.05)；给药5 d,全方组高于空白组和正常组(P＜0.05)；给药7 d,益气组高于空白组、模型组、全方组和活血组(P＜0.05)；给药14 d,活血组高于空白组、模型组、全方组和益气组(P＜0.05),空白组和正常组低于其他各给药组(P＜0.05)。结论回阳生肌膏及其拆方均能促进炎症反应,刺激炎症因子分泌,益气温阳药对创面的促炎作用早期更为明显。 　　关键词：回阳生肌膏；慢性皮肤溃疡；白细胞介素-1α；血栓素B2；大鼠 　　DOI：10.3969/j.issn.1005-5304.2014.07.013 　　中图分类号：R285.5文献标识码：A文章编号：1005-5304(2014)07-0043-04 　　Influence of Huiyang Shengji Ointment and Its Modified Formulae on Serum Interleukin-1α and Thromboxane B2 in Diabetic Rats with Chronic Skin UlcersLI Yuan1,2, ZHAO Guang-ming2, DONG Jian-xun1, LU Guang-lin2, ZHANG Xu-hui2, XU Xu-ying1, WEI Ran-ran2, CHEN Bo2 (1.Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China；2.Beijing University of Chinese Medicine, Beijing 100029, China) 　　Abstract：Objective To observe the influence of Huiyang Shengji Ointment and its modified formulae on interleukin-1 (IL-1) and thromboxane B2 (TXB2) in diabetic rats with chronic skin ulcers, and explore the mechanism for promoting the healing of ulcer. Methods Six out of 160 rats were randomly selected as a blank group, without any further processing. The remaining rats were made diabetic model and randomly divided into five groups after 2 weeks：1 d, 3 d, 5 d, 7 d and 14 d groups. Then, these groups were further divided into normal group (Vaseline ointments), model group (Vaseline ointments), Huiyang Shengji Ointment group (whole formula Ointment), Wenyang Yiqi group (Yiqi group, modified Wenyang Yiqi formula