基于代谢组学冠心病患者血瘀证识别模式研究.docVIP

基于代谢组学冠心病患者血瘀证识别模式研究 　　摘要：目的 筛选冠心病血瘀证相关的特征性代谢产物，尝试建立冠心病气虚证在代谢层面的识别模式。方法 对45例冠心病患者及15例正常人的血浆样本进行核磁共振检测，对其血浆中的内源性小分子及大分子代谢产物进行全面鉴定。对获得数据进行PLSDA、VIP及t检验/非参数检验分析，筛选出与冠心病患者血瘀证最可能相关的特征性代谢产物。 结果 检测到冠心病患者和正常对照组血浆内源性代谢成分39种。CPMG及LED代谢产物的PLSDA积分矩阵图显示：冠心病血瘀证与非气虚证的分布区沿第一主成分t（1）轴方向完全分开，模型拟合度较好。特征性代谢物筛选结果显示：血瘀证的特征性代谢产物包括缬氨酸和丙酮。结论 冠心病患者血瘀证的异常代谢过程主要包括了氨基酸代谢、脂质代谢；筛选出的2种代谢生物标志物基本上实现了对冠心病血瘀证的有效识别。 　　关键词：冠心病；血瘀证；代谢组学；识别模式 　　中图分类号：R541.4 R256.2 文献标识码：A doi:10.3969/j.issn2014.05.001 文章编号2014 　　Blood Stasis Syndrome Identification Modes of Coronary Heart Disease Based on Metabonomics 　　Shi Qi,Wang Wei,Li Youlin,et al 　　The Key Institute of State Administration of Traditional Chinese Medicine,ChinaJapan Friendship Hospital(Beijing 100029) 　　Abstract:Objective To screen the characteristic metabolites related to the blood stasis syndrome to establish blood stasis syndrome identification modes in the metabolic level.Methods Plasma samples of 45 patients with coronary heart disease (CHD) and 15 healthy subjects were detected with hydrogen nuclear magnetic resonance.The plasma endogenous metabolites were identified and screened from micro molecules to macro molecules by PLSDA,VIP and method t tests/non parametric test.Characteristic metabolites related to blood stasis syndrome were screened.Results Thirtynine endogenous metabolites were detected in CHD patients and healthy control roup.PLSDA integral matrix graphs of CPMG and LED showed:Distribution region of blood stasis and nonblood stasis patients could be completely separated along the t (1) axis direction.The separated modes had a high fitting degree.Characteristic metabolites screening results showed that characteristic metabolites of blood stasis were valine and acetone.Conclusion The abnormal metabolism in CHD blood stasis patients was amino acid and lipid metabolism.These 2 metabolic biomarkers basically could realize the effective recognition of blood stasis syndrome. 　　Key words:metabonomics; cor