基于肠道菌群代谢知母皂苷BⅡ体外代谢转化研究.docVIP

基于肠道菌群代谢知母皂苷BⅡ体外代谢转化研究 　　摘要：目的 通过大鼠肠道菌群体外实验，观察大鼠肠道菌群对知母皂苷BⅡ的代谢转化情况。方法 收集大鼠新鲜粪便，将大鼠肠道菌加入厌氧培养液得到肠道菌孵育液，加入知母皂苷BⅡ，在厌氧环境中37 ℃温孵24 h，经沉淀离心，取上清液，采用高效液相色谱和薄层色谱方法对知母皂苷BⅡ降解情况和代谢成分进行分析。结果 知母皂苷BⅡ在大鼠粪便孵育液中很快发生代谢转化，24 h后孵育液中已检测不到知母皂苷BⅡ，能检测出较高浓度的代谢产物。代谢产物经检测为知母皂苷AⅢ。结论 离体培养的大鼠肠道菌可对知母皂苷BⅡ进行代谢，初步确定代谢产物为知母皂苷AⅢ。 　　关键词：知母皂苷BⅡ；大鼠肠道菌；代谢 　　DOI：10.3969/j.issn.1005-5304.2018.05.015 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）05-0066-05 　　Study on Metabolism and Transformation of Timosaponin BⅡ in Vitro 　　Based on Metabolism of Intestinal Flora 　　LIU Yan-ping1， YU Zi-cheng2， CHEN Hong-jun2 　　1. Pudong New Area People’s Hospital， Shanghai 201200， China； 　　2. Yangpu Hospital of Tongji University， Shanghai 200090， China 　　Abstract： Objective To observe metabolic transformation of Timosaponin BⅡ in intestinal flora of rats by rat intestinal flora in vitro. Methods After the fresh rat feces were collected， the intestinal bacteria were added to the anaerobic medium to obtain the intestinal bacteria incubation fluid. Timosaponin BⅡ was added and cultured， and incubate at 37 ℃ in anaerobic environment for 24 h. Then samples were gathered through deposition and centrifugation. The supernatant was analyzed by HPLC and TLC method， and the degradation rate and metabolic components of Timosaponin BⅡ were analyzed. Results In vitro， Timosaponin BⅡ was metabolized by the intestinal flora soon and it could not be detected in the incubation fluid after 24 h， which could detect a higher concentration of metabolites. The metabolites preliminary identified as Timosaponin AⅢ. Conclusion Rat intestinal flora in vitro have metabolism effect on Timosaponin BⅡ. The metabolites are initially identified as Timosaponin AⅢ. 　　Keywords： Timosaponin BⅡ； intestinal bacteria； metabolize 　　知母?榘俸峡浦参镏?母Anemarrhena asphodeloides Bge.的干燥根茎，味苦、性寒，具有清热泻火、止渴除烦、滋阴润燥等功效，临床用于高热烦渴、外感热病、内热消渴、肺热燥咳、骨蒸潮热、肠燥便秘等。知母皂苷BⅡ（Timosaponin BⅡ，TBⅡ）是知母中的主要活性成分，具有抗老年痴呆、改善学习记忆、抗抑郁、抗肿瘤、抗炎、降糖等药理活性[1-2]。 　　基金项目：上海市杨浦区科技委卫计委科研课题（YP15Q05）；同济大学青年优秀