uPA合成抑制剂Amiloride对人宫颈癌细胞体外侵袭迁移及凋亡影响-妇产科学(妇科肿瘤学)专业论文.docxVIP

8 8 Amiloride incubation were significantly lower than the control group (F=56.893, 360.000,360.038,all P0.01), and the migration was positively correlated with the uPA mRNA expression levels(r=0.942, P0.01). The number of Hela cells that penetrated the Matrigel after 50,100,150μmol/L Amiloride incubation decreased obviously in comparison with control group (F=226.95,P0.01),and the invassiveness was positively correlated with the uPA mRNA expression levels(r=0.969, P0.01). The early apoptosis rate of Hela cells after 50,100,150μmol/L Amiloride incubation 24,48 hours increased obviously in comparison with control group (F=59.895,472.428,all P0.01), and the apoptosis rate was negatively correlated with the uPA mRNA expression levels(r=﹣0.941, P0.01). Conclusions: The low concentration of Amiloride could specificly inhibit the expression of uPA mRNA of human cervical carcinoma Hela cell lines in a concentration - time dependence, what could cause decrease of the expression of MMP-2 mRNA which was the uPA system’s downstream factor, but do no effect to uPAR and Matriptase which was the uPA system’s upstream regulator. uPA may be a gene associated with the invasion and metastasis of human cervical cancer, Amiloride can suppress the invasion and metastasis of Hela cells in vitro by down-regulation the uPA mRNA expression. uPA may be a gene associated with cell apoptosis, Amiloride can induce apoptosis of Hela cells in vitro by down-regulation the uPA mRNA expression, suggesting that deserves further study about its anti-tumor effect. Keywords amiloride; cervical cancer; urokinase-type plasminogen activator; invasion and metastasis; cell apoptosis 4 4 英文缩略词表 英文缩写 英文全称 中文名称 uPA urokinase-type plasminogen activator 尿激酶型纤溶酶原激活剂 urokinase-type plasminogen activator 尿激酶型纤溶酶原激活剂 uPAR receptor 受体 urokinase-type plasminogen activator 尿激酶型纤溶酶原激活剂 PAI inhibitor 抑制因子 FN fibronectin 纤维粘连蛋白 DMSO dimethyl sulphoxide 二甲基亚砜 MMPs Matrix Metalloproteinases 基质金属蛋白酶 EDTA ethylenediaminotetraa