不同道地产区木瓜指标成分分析及川木瓜总黄酮提取纯化工艺分析-药物化学专业论文.docxVIP

Ursolic acid and oleanolic acid as reference substance were determined by RP-HPLC method.The result is that the content of different samples are different. A spectrophotometric method was established for quantitative determination the total content of flavonoids in chaenomeles (Sweet) Nakai. The result showed that the optimal determination wavelength was 510nm. There was a good linear relationship between the absorbance and the quantity of rutin when the concentration of rutin(contrast) within the range of 4.64ug~27.84ug/ml.Regression equation was Y=1.0998X-0.0073 with the correlation coeffcient of 0.9991. The average recovery rates were 97.84%. This method is stable and precise as well as high recovery rates. We determine the total flavonoids content of eight different sourced mugua. The result is that the content of total flavonoids from Sichuan is as much as Anhui. 4. The general inspection to the different habitat raw material for medicine has been carried, finally indicated raw material for medicine all conforms to the pharmacopoeia stipulation standard. 2.The extraction, purification of flavonoids in Chuan mugua. The extraction process of the total flavonoids in Chuan mugua was investigated by single factor and orthogonal experiment. The optimum condition for the extraction of the total flavonids in it was that alcohol concentration is 80%; temperature is 60C;extraction time is three and one hour of each and the ratio of solid to liquid is 1:10. The column Chromatography was employed to enhance the content of the total flavonoids. In terms of the result of static adsorption and static de-adsorption test, D140 resin was the best one for isolation flavonoids. The optimal adsorption parameters were feeding rate 1 BV/h, pH value 5~6,medicine liquid concentration 0.1mg/ml. The optimal de-adsorption parameters were eluted with distilled water, 3BV 10% ethanol and 3BV 50% ethanol in turn, flow rate 2BV/h. The purified total flavonoids was collected in 50% ethanol elu