尼莫地平对AD大鼠脑组织NO影响.docVIP

尼莫地平对AD大鼠脑组织NO影响 　　[摘要] 目的 探讨尼莫地平改善痴呆大鼠模型学习记忆能力的机制。方法 随机将30只大鼠分为三组,空白对照组不作处理,模型组和尼莫地平组用Aβ25～35脑海马注射造模,尼莫地平组给予尼莫地平干预。2周后进行迷宫实验,比较各组大鼠行为上的差异。3周后脑组织NO检测,进行组间比较。结果 尼莫地平组学习记忆能力优于模型组,三组脑组织NO含量,经方差分析有显著性差异(P0.01)。结论 尼莫地平能改善痴呆大鼠模型的学习和记忆能力,这种改善可能与维持Ca2+稳态、保护脑组织、减少NO等神经毒损害有关。 　　[关键词] 阿尔茨海默病; 尼莫地平; 一氧化氮 　　[中图分类号] R749.16 [文献标识码] A [文章编号] 1673-9701(2010)07-05-03 　　 　　Effect of Nimodipine on AD Rat Brain Tissue NO 　　GAO Lu1 WANG Chaowei1 HU Weimin2 　　1.Shanxi Medical University,Taiyuan 030001,China;2.Department of Neurology,the Second Clinical Medical College of Shanxi Medical University,Taiyuan 030001,China 　　 　　[Abstract] ObjectiveTo explore the mechanism of whether nimodipine can improve learning and memory in dementia rat model. Methods Thirty rats were randomly divided into three groups:control group with no treatment,the model group and nimodipine group. The dementia rat model was established by injection of brain hippocampus Aβ25～35,and the nimodipine group was given nimodipine intervention. The maze test was carried out after two weeks and the comparison was made of the differences in rat behavior of the three groups. The brain tissue NO content was detected after three weeks and the comparison was made in the three groups. ResultsBoth learning and memory of the nimodipine group were better than those of the model group. The NO content in the brain tissue of the three groups was detected,and the analysis of variance showed a significant difference(P0.01). ConclusionNimodipine can improve learning and memory of dementia rat,which may be related to the maintenance of Ca2+ homeostasis and protection of brain tissue,such as reduction of NO neurotoxicity damage. 　　[Key words]Alzheimer disease; Nimodipine; NO 　　 　　1994年Khachaturian提出了阿尔茨海默病(Alzheimer disease,AD)的钙稳态学说[1],认为细胞Ca2+ 浓度的持续升高会导致神经细胞损伤和凋亡。细胞培养和动物实验证明,Ca2+稳态失衡与AD病理特点有关[2]。高Ca2+可活化氧化氮合酶,产生NO。NO是一种自由基,在中枢神经系统参与多种病理和生理作用。实验证明,海马内NO的表达和学习记忆密切相关。血管性痴呆时脑脊液中NO浓度与痴呆程度呈正相关。尼莫地平(NIM)是二氢吡啶类钙拮抗剂,动物实验证明,它能增加动物的探究行为、改