尼莫地平对MCAOR模型大鼠不同时间さ阊流灌注量影响.docVIP

尼莫地平对MCAOR模型大鼠不同时间さ阊流灌注量影响 　　【摘要】目的：利用激光散斑血流成像技术观察尼莫地平对大鼠大脑中动脉栓塞模型脑血流灌注量的影响。方法：成年雄性SD大鼠，采用改良线栓法制备大脑中动脉梗塞/再灌注模型。利用激光散斑血流成像技术观测造模前，缺血再灌注05、1、2、24h后脑血流灌注量情况，并对选定区域血流进行分析，再灌注24h TTC染色观察脑梗死体积。结果：造模成功后大鼠右侧脑血流明显减少，灌胃给4mg/kg尼莫地平后，大鼠局部脑血流渐渐增加，脑梗死面积减少，脑组织含水量减少。结论：尼莫地平能显著增加MCAO/R模型大鼠血流量。 　　【关键词】尼莫地平；脑缺血再灌注损伤；激光散斑血流成像；脑血流量 　　【中图分类号】R965【文献标志码】 A【文章编号】1007-8517（2017）07-0042-04 　　Effect of Nimodipine on MCAO/R Rats Different Time Point Blood Perfusion 　　ZHANG Zhixue1，3HUANG Xi1，3LI Yue1，2，3YANG Zhibin1，2，3XIAO Huai1，2，3WU Xiumei1，2，3ZHANG Chenggui1，2，3* 　　1.Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical RD， Institute of Entomoceutics Research， Dali University， Dali 671000， China；2.Yunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics， Dali University，Dali 671000， China；3.National-Local Joint Engineering Research Center of Entomoceutics，Dali 671000， China 　　Abstract：Objective To study the effect of nimodipine on cerebral blood flow in MCAO/R rats by laser speckle imaging （LSI）. Methods To establish the MCAO/R model in male SD rats by the modified intraluminal filament technique. Measuring the cerebral blood flow before modeling and after ischemia-reperfusion for 05 h， 1 h， 2 h， 24 h by LSI， meanwhile analysing the selected area blood flow， and observing the infarct size after perfusion for 24 h with TTC. Results The cerebral blood flow was decreased in right hemisphere after the models established successfully， and after the intragastric administration of nimodipine with 4 mg/kg， the regional cerebral blood flow of rats gradually increased， the cerebral infarction area reduced， and the brain water content was decreases. Conslusions Nimodipine significantly improve the cerebral blood flow of MCAO/R rats. 　　Keywords：Nimodipine； Cerebral Ischemia-Reperfusion Injury； Laser Speckle Imaging； Cerebral Blood Flow 　　缺血性?X卒中（Ischemic Stroke，IS）指脑的供血主动脉发生狭窄或阻塞，脑的血液供应出现障碍，导致氧和营养物质的缺乏，从而引起脑的功能异常，脑局部组织坏死[1]。局灶性脑缺血后大脑损伤的进展取决于血流量减少的严重程度和缺血后再灌注的动力学