不同离子通道调节剂对茶树吸收富集F的Ca2+信号转导机制的分析-茶学专业论文.docx

安徽农业大学博士论文英文摘要 安徽农业大学博士论文 英文摘要 万方数据 万方数据 Abstract Tea plants are hyper-accumulators of fluoride, and quantities of fluoride accumulated in the tea plants were 20-30 times higher than that in other plants. About 40–90% of fluoride in commodity tea can dissolve in tea liquid. Drinking tea is an important approach to ingest fluoride in the human body. Ingestion of fluoride from drinking tea containing normal fluoride concentrations has been considered safe and good to health. But ingestion of excessive fluoride resulted in chronic fluorosis, which was known as dental fluorosis and skeletal fluorosis. Recently, the study of fluoride in tea plants mainly focus on the influencing factors and distribution pattern of fluoride in tea plant, but the absorption pathways and mechanisms of fluoride absorption in tea plant remains unknown. In this paper, the ways by which fluoride entering the tea plants and Ca2+ signal transmission approaches in tea plants were investigated, and results from this work will provide critical facts for clarifying characteristics of fluoride absorption and accumulation in tea plants. The effects and micro-mechanisms of different ion channel inhibitors on the absorption and accumulation of fluoride in tea plants were studied.The concentration of Ca2+ in cytoplasm and Ca2+ fluxes through the plasma membranes were measured using the confocal laser scanning microscopy(CLSM) and non-invasive micro-test technique on the elongation zone of tea roots(NMT); The total and plasma membrane Ca2+-ATPase activities and calmodulin (CaM) contents on tea roots were determined by using the enzyme method and enzyme - linked immunosorbent assay(ELISA). The enzyme activities analytical method coupled with non-invasive micro-test technique were explored to determine the activities of the total H+-ATPase and plasma membrane H+-ATPase, as well as the H+ fluxes through the plasma membranes and membrane potential. The influences of different ion channel inhibitors on Ca2+ sig