微柱凝胶技术用于新生儿溶血病检测中价值分析.docVIP

微柱凝胶技术用于新生儿溶血病检测中价值分析 　　[摘要] 目的 对微柱凝胶技术用于新生儿溶血病（HDN）检测中的价值进行探讨分析。方法 选取本中心2013年1月～2017年1月期间收集的80份HDN患儿血液标本，以此作为本次研究的观察对象，使用传统试管法和微柱凝胶技术分别进行抗体放散试验、抗体游离试验及直接抗人球蛋白试验，对所取得了检测结果进行对比。结果 通过对80份HDN患儿血液标本的检测，采用试管法进行抗体放散试验检测出67份（83.75%）呈阳性，进行抗体游离试验检测出62份（77.50%）呈阳性，进行直接抗人球蛋白试验检测出54份（67.50%）呈阳性，而采用微柱凝胶技术进行抗体放散试验检测出76份（95.00%）呈阳性，进行抗体游离试验检测出72份（90.00%）呈阳性，进行直接抗人球蛋白试验检测出70份（87.50%）呈阳性，试管法和微柱凝胶技术在三种试验中的检测阳性率差异具有统计学意义（P0.05）。结论 临床针对新生儿溶血病可采用微柱凝胶技术进行检测，其具有较高的敏感性，结果稳定、操作简单，能为临床治疗提供有利依据，具有较高的临床应用价值，值得推广使用。 　　[关键词]新生儿溶血病；微柱凝胶技术；检测价值 　　[中图分类号] R722.1 [文献标识码] A [文章编号] 2095-0616（2017）14-49-03 　　Value analysis of microcolumn gel technique in detection of hemolytic disease of the newborn 　　REN Jun 　　Machine Generated Branch， Guangzhou Blood Center， Guangzhou 510095， China 　　[Abstract] Objective To explore the value of microcolumn gel technique for hemolytic disease of the newborn （HDN）. Methods 80 HDN children with blood samples from January 2013 to January 2017 in our center were collected as the research object of the observation. The traditional tube method and microcolumn gel technique were used to perform antibody elution test， free antibody test and direct antiglobulin test respectively. The test results were compared. Results Among the HDN of 80 children with blood samples for antibody detection， elution test detected 67 samples by test tube method （83.75%）. Positive antibody free test showed 62 （77.50%） were positive. For direct antiglobulin test， 54 samples （67.50%） were positive， while using microcolumn gel technique for antibody elution test，76 samples （95%） were positive. For antibody free test detected 72 copies （90%） positive. Direct antiglobulin test detected 70 samples （87.50%） were positive in the test tube method and microcolumn gel technique in three test in the detection positive rate difference （P0.05）. Conclusion The clinical for hemolytic disease of the newborn can be used Micro column gel technology for detection. It has high sensitivity， stable results， and