滋肾丸治疗去卵巢雌鼠骨质疏松实验分析滋肾丸治疗绝经后骨质疏松的临床观察-experimental analysis of zishen pill in treating osteoporosis in ovariectomized female rats clinical observation of zishen pill in treating postmenopausal osteoporosis.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 2 中文摘要 目的:探讨中药滋肾丸对去卵巢骨质疏松模型大鼠激素水平的改善作用及机制研 究。 方法:将 66 只 7 月龄 SD 大鼠随机分为 6 组，每组 11 只，空白组做假手术，其余 5 组做卵巢切除术，术后 2 周开始给药，用药 12 周后取血测定血清促性腺激素释放激 素、促卵泡激素和促黄体生成素含量，处死后取子宫称重,取股骨远端 1/3 部分及胫 骨近端 1/3 部分进行骨组织形态学研究。 结果:模型组的血清促性腺激素释放激素、促卵泡激素和促黄体生成素均有升高, 子宫指数降低, 骨组织退化，出现骨小梁数目减少，面积百分率下降。中药滋肾丸大、 中、小剂量组及尼尔雌醇组均能使促性腺激素释放激素、促卵泡激素和促黄体生成素 降低,子宫指数升高,骨小梁数目增加 ，面积百分率上升，差异具有统计学意义 （P0.05)。 结论:中药滋肾丸对去卵巢骨质疏松大鼠的促性腺激素释放激素、卵泡刺激素、 促黄体生成素有降低作用，对骨组织形态学有改善作用,使其骨小梁数目增加，面积 百分率上升。其疗效可能与其延缓性腺轴衰老，提高雌激素水平,从而降低破骨细胞 的活性、增加成骨细胞的活性有关，表明中药滋肾丸对骨质疏松有一定的治疗作用。 关键词:骨质疏松 性腺轴 中药 实验研究 滋肾丸治疗去卵巢雌鼠骨质疏松实验研究——滋肾丸治疗绝经后骨质疏松临床观察 滋肾丸治疗去卵巢雌鼠骨质疏松实验研究——滋肾丸治疗绝经后骨质疏松临床观察 Abstract Objective To investigate the effet and the mechanism of kidney-reinforcing Chinese herb Zishenwan on osteoporosis of ovariectomized rats. Method Female SD rat (n=66,7monthes old) were randomly assigned to 6 groups of 11 eaeh.The blank group weer shamming operated,the other groups weer performed surgery of removal both ovary.Medicines were given 2 weeks after surgery,which last 12 weeks and then killed. The blood were collected,Gn-RH,FSH and LH weer determined, the womb were taken out,and be weighted. After executing take the outlying l/3 quarters of thighbone and close l/3 quarters of shinbone,investigate the tectology of bone. Result The level of Gn-RH,FSH and LH increased and the weight of womb decreased vidently in model group, lumber number of bone significantly reduce,the bone organization degraded,the number of trabecula reduced,the thickness become thin,the area percent declined. In Zishenwan group including big,middle,small dosage group and nylestriol group ,the weight of womb increased,the level of Gn-RH,FSH and LH decreased,the number of trabecula increased,the thickness thickened,the area percent raised,which has the statistics meaning(p＜0.05). Conclusion Traditional Chinese medicine Zishenwan has reduced the Gn-RH、 FSH and LH, improved the bone organization form of the