中国美利奴绵羊mhc class i区段429p24 bac克隆序列组成与结构研究-cloning sequence composition and structure of 429 p24 bac in mhc class i segment of chinese merino sheep.docxVIP

IV IV cDNA clones using BAC probes is effective and practical. The result revealed that these genes maybe related to immune and disease of sheep. 3. Through the sequence obtained with the 429P24 BAC clone sequence alignment, results showed that the gene is indeed derived from Chinese Merino sheep MHC ClassI section of the gene. Conclusion: To a large extent biological study is can not to break away from bioinformatics. Applying the bioinformatics methods can make our study better. When running into some complicated experiment question, especially about Genome sequence construction and functional study, bioinformatics is more important. Experiment results also indicated that using bioinformatics methods can more effective and shortcut to obtain the express genes. Furthermore this method is very simple and technique is very maturation. It is a feasibility methods of bacteriophage hybridization in situ in the hybridizate of library and library. Especially study some low express genes. This methods obtains the research worker’s favor for it’s high sensitivity, operation simple and short cycle. Experiment results also indicated that this method is feasibility. Key word: labeled with 32P; sheep; MHC; cDNA library; probe VII VII 英文缩写词表 英文缩写 英文全称 中文名称 Amp Ampicillin 氨苄青霉素 MHC major histocompatibility complex 主要组织相容性复合体 dNTP deoxyribonucleoside trephosphate 脱氧核苷三磷酸 Gene Bank gene bank 核苷酸序列资料库 IPTG Tsopropythis-a-D-galactoside 异丙基硫代-a D-半乳糖苷 mmol/l mill mole per liter 毫摩尔/升 mol/l mole per liter 摩尔/升 OD Optical density 光密度 ORF Open reading frame 开放阅读框 pH pH value pH 值 r/min revolution per minute 转/分钟 Tris hydroxymethyl aminomethans 三羟甲基氨基甲烷 TBE Tris/Borate/EDTA Tris 缓冲液 Taq DNAase Taq DNA polymerase Taq DNA 聚合酶 TE Tris-Cl-EDTA buffer TE 缓冲液 U unit 单位 μl microliter 微升 5-broma-4-chloro-3-indoly-a-D-gala X-gal  ctoside 5-溴-4-氯-3-吲哚-a-D-半乳糖苷 石河子大学学位论文独创性声明及使用授权声明 学位论文独创性声明 本人所呈交的学位论文是在我导师的指导下进行的研究工作及取得的研究成果。据我所知，除 文中已经注明引用的内容外 ，本论文不包含其他个人已经发表或撰写过的研究成果。对本文的研究 做出重要贡献的个人和集体，均已在文中