液质联用分析D―GlaN致急性肝损伤模型大鼠尿液中肋柱花化学成分.docVIP

液质联用分析D―GlaN致急性肝损伤模型大鼠尿液中肋柱花化学成分 　　【摘 要】 目的：研究肋柱花水煎液在D-GlaN致急性肝损伤模型大鼠尿液中的化学成分。方法：采用腹腔注射D-GlaN制备急性肝损伤大鼠模型；利用液质联用法对模型大鼠灌胃肋柱花水煎液后尿液中化学成分进行分析，检测波长280nm；流动相为甲醇-01%甲酸水溶液；流速10ml/min；柱温35℃；梯度洗脱，质谱定性采用多级离子阱质谱，负离子模式扫描。结果：在含药尿液中共标识19个峰，1个为药物原型成分，14个为代谢产物，其余为尿液内源性成分。结论：该方法较全面的分析肋柱花在急性肝损伤模型大鼠尿液中的化学成分，为进一步研究肋柱花药效物质基础提供依据。 　　【关键词】 肋柱花；液质联用；急性肝损伤模型；尿液 　　【中图分类号】R2841 【文献标志码】 A 【文章编号】1007-8517（2015）05-0012-03 　　Chemical composition analysis of Herba Lomatognii in the urine from ratmodel of D-GlaN induced acute liver injury by LC-MS 　　TONG Yufeng，BA Genna，BAI Meirong，BAO Minglan，YE Rigui 　　Inner Mongolia University for the Nationalities，TongLiao 028000，China 　　Abstract：[WTHZ]Objective[WTBZ] To analyze the chemical composition of Herba Lomatognii water decoction in the urine from rat model of D-GlaN induced acute liver injury.[WTHZ]Methods[WTBZ] Intraperitoneal injected D-GlaN to prepare rat model of acute liver injury；Analyze the chemical composition of the urine after the rats were given Herba Lomatognii water decoction by gavage；The detection wavelength was 280nm；the mobile phase was methanol and water；the flow rate was 10ml/min；column temperature was 35℃；gradient elute.[WTHZ]Results[WTBZ] 19 compositions were detected in the Medicated urine，1 ones were drug-induced compositions，14 ones were metabolites，and others were the natural ingredients for urine.[WTHZ]Conclusion[WTBZ] This method comprehensively analyzed the chemicalcomposition of Herba Lomatognii in the urine from rat model of D-GlaN induced acute liver injury. This research is a theoretical indication for the further research on effective substance of Herba Lomatogonii. 　　Keywords：Herba Lomatogonii；LC-MS；Acute liver injury model；Urine 　　肋柱花为龙胆科肋柱花属植物肋柱花Lomatogonium rotatum（L）Fries ex Nym的干燥全草。广布在我国西藏、云南、四川、青海、甘肃、新疆、内蒙、山西和河北。该药是蒙医特色药材，具有平息协日、清热、健胃、愈伤之功。主要用于清协日热、瘟疫、流感、伤寒、中暑头痛、肝胆热、黄疸、胃协日、伤热等病症[1]。1998年收载于卫生部药品标准（蒙药分册）。近年来，研究资料显示，肋柱花所含黄酮类化合物、木犀草素、齐敦果酸等均具有不同程度的利胆、保肝活性[2]，在一定程度上提示肋柱花可用于治疗肝胆疾病。 　　课题组对该药材乙酸乙酯、氯仿、正丁醇及石油醚提