清热化湿法对非酒精性脂肪性肝炎大鼠影响.docVIP

清热化湿法对非酒精性脂肪性肝炎大鼠影响 　　摘要：目的：通过高脂饮食建立大鼠非酒精性脂肪性肝炎模型，观察清热化湿方王氏连朴饮加减方对其血清生化和肝组织病理的影响。方法：采用普通饲料加10%猪油、2%胆固醇高脂饮食复制大鼠NASH模型。实验分正常对照组、空白模型组、清热化湿方防治组、东宝肝泰（复方蛋氨酸胆碱片）对照组。测定各组血清甘油三酯(TG)、总胆固醇（TCH）、低密度脂蛋白（LDL）和丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平，评估肝组织病理脂肪变性和炎症活动程度。结果：大鼠NASH模型成功建立。与空白模型组相比，东宝肝泰对高脂饮食诱导的NASH大鼠具有降低TG、 TCH、LDL作用（P0.05）；清热化湿方防治组血清TG、TCH、LDL、ALT、AST明显降低（P0.01），作用均优于东宝肝泰对照组（P0.05），清热化湿方防治组脂肪变性和炎症活动程度明显改善（P0.01），其炎症活动程度较东宝肝泰对照组明显减轻（P0.05）。结论：清热化湿方对NASH有良好的防治作用。部分作用优于东宝肝泰组（P0.05）。 　　关键词：非酒精性脂肪性肝炎；动物模型；组织病理；清热化湿法 　　中图分类号：R285.5文献标识码：A文章编号：1673-7717(2012)03-0564-03 　　Influence Of Heat-Clearing And Dampness-Resolving Method On 　　Rats With Non-Alcoholic Steatohepatitis 　　LIU Lin1， YANG Qing?fang1，YAN Hong?mei2,ZHANG Chi?zhi3 　　(1. Hubei University of Traditional Chinese Medicine，Wuhan 430061，Hubei，China； 　　2. Wuhan No 7 Hospital，Wuhan 430064，Hubei，China；3.Hubei University of 　　Traditional Chinese Medicine，Wuhan 430061，Hubei，China) 　　Abstract:Objective:The rat model of non-alcoholic steatohepatitis was established by high fat diet. The influence of modified Lianpu Decoction on the biochemistry changes and liver tissue pathology changes was observed. Methods:NASH model was duplicated by 2% common forage with 10% lard and 2% high fat diet. There was normal control group, blank control group, heat-clearing and dampness-resolving group, Dongbao Gantai control group. Levels of TG, TCH, LDL, ALT and AST in each group were measured. The fatty degeneration of liver tissue and the activity of inflammation were evaluated. Results:The NASH model was successfully established. Compared with the blank control group, Dongbao Gantai can reduce the levels of TG, TCH and LDL in NASH rats induced by high fat diet（P0.05）. The heat-clearing and dampness-resolving group can obviously reduce the levels of TG,TCH,LDL,ALT and AST（P0.01）, the effect was better than the Dongbao Gantai control group（P0.05）, and it can obviously improve the fatty degeneration and inflammation activity（P0.01）, the inflamm