生发酊对C57BL6小鼠毛囊细胞凋亡影响.docVIP

生发酊对C57BL6小鼠毛囊细胞凋亡影响 　　[摘要] 目的 从毛囊细胞凋亡的角度出发，观察生发酊对动物模型小鼠毛发生长周期的影响，评价其治疗脱发的效果。 方法 将C57BL/6小鼠随机分为生发酊组、阳性对照组、阴性对照组、模型对照组，制备小鼠脱发动物模型。脱毛后第2天，于脱毛区涂相应药物；脱毛后第17天，颈椎脱臼处死小鼠，背部平行脊柱相同部位取材，用原位杂交技术原位末端标记法（TUNEL）检测毛囊内细胞的凋亡状况。 结果 生发酊组[（15.82±0.36）个/视野]与阳性对照组[（16.12±0.39）个/视野]细胞凋亡数量明显少于阴性对照组[（40.32±0.47）个/视野]与模型对照组[（42.16±0.54）个/视野]，差异有统计学意义（P0.05）。 结论 生发酊对毛囊细胞凋亡有明显的抑制作用，此为其治疗脱发的可能机制之一。 　　[关键词] 生发酊；细胞凋亡；毛囊细胞 　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1674-4721（2016）04（c）-0122-04 　　Impact of hair-growth tincture on hair follicle cell apoptosis in animal model for C57BL/6 mice 　　ZHANG Chun-hong 　　Department of Dermatology，Changchun Hospital of Traditional Chinese Medical，Changchun 130022，China 　　[Abstract] Objective To observe the impact of hair-growth tincture on mice hair growth cycle in animal model from the angle of hair follicle cell apoptosis and to evaluate its effects in treatment hair loss. Methods C57BL/6 mice were randomly divided into hair-growth tincture group，positive control group，negative control group and model control group and then mice hair loss model were made.Corresponding drugs were smeared to hair loss area from the second day after hair loss.Mice were killed by cervical dislocation at the 17 day after hair loss.The samples were draw from same part of back parallel spine.In situ hybridization TUNEL was used to test the apoptosis condition of hair follicle cell. Results The apoptosis number in hair-growth tincture group and positive control group with a view of （15.82±0.36） cells and （16.12±0.39） cells were obvious less than those in negative control group and model control group with a view of （40.32±0.47） cells and （42.16±0.54） cells，and the differences were statistical significant （P0.05）. Conclusion Hair-growth tincture has obvious role in inhibiting hair follicle cell apoptosis and this may be one of the mechanism in treating hair loss. 　　[Key words] Hair-growth tincture；Cell apoptosis；Hair follicle cell 　　脱发是一种常见的疾病，在人群中的发病率较高，其发病机制尚不完全清楚。部分脱发具有先天遗传性，后天可能