组织芯片技术用于检测120例胃腺癌患者EcadherinCEA和HER2表达临床意义.docVIP

组织芯片技术用于检测120例胃腺癌患者EcadherinCEA和HER2表达临床意义 　　摘 要 目的：检测胃腺癌患者中E-cadherin、CEA和HER-2的表达，分析其在判断预后的意义。方法：选取胃腺癌患者的手术胃癌组织蜡块标本120例为观察组，手术远端正常胃粘膜组织26例为对照组，采用组织芯片技术检测E-cadherin、CEA和HER-2的表达。结果：观察组CEA及HER-2的表达高于对照组（P0.05）；E-cadherin的表达与组织学类型有关，CEA和HER-2的表达与组织学类型及远处转移有关，差异具有统计学意义（P0.05）；3年生存期患者的E-cadherin、CEA、HER-2表达均明显高于5年生存期患者，差异具有统计学意义（P0.05）。结论：采用组织芯片技术联合检测胃腺癌组织中E-cadherin、CEA和HER-2的表达，可用于判断患者的病情发展及预后，并为诊断和治疗提供一定的依据。 　　关键词 组织芯片 E-cadherin CEA HER-2 胃腺癌 　　中图分类号：R735.2； R730.7 文献标识码：A 文章编号：1006-1533（2015）23-0061-03 　　Clinical significance of the application of tissue microarray technology in the detection of the expression of E-cadherin， HER-2 and CEA in 120 cases of patients with gastric cancer* 　　LIAN Huojun** 　　（The People’s Hospital of Shangrao City， Shangrao 334000， China） 　　ABSTRACT Objective： To detect the expression of E-cadherin， CEA and HER-2 in gastric cancer patients， and analyze the prognostic significance. Methods： One hundred and twenty cases of tissue paraffin block specimens from patients with gastric cancer were selected as an observation group and 26 cases of distal surgical normal gastric mucosa as a control group. The expression of E-cadherin， CEA and HER-2 was detected by tissue microarray technology. Results： The expression of CEA and HER-2 in the observation group was higher than that in the control group （P0.05）. The expression of E-cadherin was related to histological type while the expression of CEA and HER-2 was related to not only histological type but also distant metastasis （P0.05）. The expression of CEA， HER-2 and E-cadherin in the 3 years survivals were significantly higher than that in the 5 years survivals （P0.05）. Conclusion： The expression of E-cadherin， CEA and HER-2 in gastric carcinoma detected by tissue chip technology can be used to evaluate the condition and prognosis of patients and provide a basis for diagnosis and treatment. 　　KEY WORDS tissue microarray； E-cadherin； CEA； HER-2； gastric adenocarcinoma 　　胃癌是全世界最常见的恶性肿瘤之一，由