精子DNA损伤对体外受精―胚胎移植助孕结局影响分析.docVIP

精子DNA损伤对体外受精―胚胎移植助孕结局影响分析 　　【摘要】目的 分析精子DNA损伤对体外受精-胚胎移植助孕结局的影响。方法 选择2016年2月～2017年2月在我院接受体外受精-胚胎移植助孕的男性患者355例，收集其资料进行回顾性分析，按照精子损伤是否超过25%，分成精子DNA损伤组（观察组）125例，未损伤组（对照组）230例，分析组间受精、可移植胚胎、优质胚胎、妊娠及流产情况。结果 观察组精子浓度、活力、正常形态精子率、受精率、可移植胚胎率、优质胚胎率等检测指标均低于对照组，差异有统计学意义（P0.05）；观察组妊娠率低于对照组，差异有统计学意义（P0.05），而流产率则高于对照组差异有统计学意义（P0.05）。结论 精子DNA损伤能够对体外受精-胚胎移植助孕结局造成一定负面影响，因此加强精子DNA损伤的检测，有助于预测体外受精-胚胎移植助孕结局。 　　【关键词】精子DNA损伤；体外受精-胚胎移植；助孕结局 　　【中图分类号】R321 【文献标识码】B 【文章编号】ISSN.2095-6681.2017.32..03 　　【Abstract】Objective To investigate the effect of sperm DNA fragmentation index on the pregnancy outcome after in vitro fertilization （IVF） procedure.Methods We selected 355 male patients who underwent conventional IVF-ET in our hospital between 2016.2 and 2017.2.A retrospective analysis was performed with collected data. Patients were divided into sperm DNA damage group （observation group） 125 cases， non-damage group （control group） 230 cases according to the sperm DNA fragmentation index was/wasnot more than25%.Fertilization rate， the number of transferable embryos，high-quality embryo rate，pregnancy rate and abortion rate were recorded for statistics analysis.Results The observation group in sperm concentration， motility， normal sperm morphology rate， fertilization rate， detection index of embryo transfer rate and good quality embryos rate were lower than the control group， the difference was statistically significant （P0.05）； pregnancy rate in observation group than in the control group， the difference was statistically significant （P0.05）， while the abortion rate is higher the control group was statistically significant （P0.05）.Conclusion Sperm DNA damage may have some negative effects on the outcome of IVF， thus the detection of sperm DNA fragmentation index is conducive to predicting the outcome of IVF. 　　【Key words】Sperm DNA fragmentation；in vitro fertilization-embryo transfer；pregnancy outcome 　　近年?恚?随着生活压力的增大，环境的污染及高龄夫妇越来越多，导致不孕不育的夫妇越来越多。因此，越来越多的夫妇采取体外受精-胚胎移植技术助孕。据文献报道，其中因男性因素引起的不孕不育症约占2