胃癌淋巴结浸出液癌胚抗原含量在淋巴结微转移中意义.docVIP

胃癌淋巴结浸出液癌胚抗原含量在淋巴结微转移中意义 　　摘要：目的了解胃癌淋巴结浸出液中癌胚抗原含量在评估胃癌淋巴结微转移中的临床意义。方法对2012年4月~2013年4月收入院的71例行D2胃癌根治术患者，应用电光化学法测量清扫的淋巴结浸出液癌胚抗原含量，同常规HE染色法、免疫组化法检查进行对比分析。结果同常规HE染色法进行对比分析显示，电光化学法测量癌胚抗原含量检测淋巴结转移在敏感度方面高于常规HE染色法，P0.05，有统计学意义，同免疫组化法对比分析，两种方法在敏感度方面无统计学差异。结论根据胃癌术后淋巴结浸出液癌胚抗原含量判断淋巴结微转移的敏感度优于HE染色法，有统计学意义。同免疫组化法无统计学差异。在指导术后早期应用辅助治疗及PN方面有一定的意义。 　　关键词：胃癌；淋巴结浸出液；癌胚抗原；电光化学法；HE染色法 　　Clinical Significance of Carcinoembryonic Antigen Level in Lymph Node Leaching Solution with Lymph Node Micrometastasis 　　ZHAO Chuan-jie,LIU Mao-rong,LI Shu-liang,LI Pei-zhe 　　(Deparment of Gastrointestial Surgery,The Second People’s Hospital of Liaocheng City,Linqing 252600,Shandong,China) 　　Abstract：Objective To assess the clinical significance of carcinoembryonic antigen level in lymph node leaching solution with lymph node micrometastasis. Methods Patients who underwent the radical operation of gastric cancer from 2012 April to 2013 April were included in this experiment. We use the electro-optic chemical detection to detect the CEA content in leaching solution of lymph node in order to comparative analyze with routine HE staining and immuneohistochemistry. ResultsThe results in the electro-optic chemical method has a higher sensitivity than that in the conventional HE staining(P0.05).However,there is also no statistical difference between the results in electro-optic chemical and immunohistochemistry method about sensitivity. ConclusionCompared with the conventional HE staining method, to detect the carcinoembryonic antigen level in lymph node leaching solution with electro-optic chemical method has a higher sensitivity to assess the lymph node micrometastasis. However,there is no statistical difference with immunohistochemistry. As a result, it is valuable to do eary adjuvant treatment and make PN stage in clinic. 　　Key words：Gastric cancer;Leaching solution of lymph nodes;CEA;Electro-optic chemical method;Hematoxylin-eosin staining胃癌淋巴结转移是影响胃癌术后预后最主要的原因之一。研究表明，胃癌转移是在微转移的基础上发展而来，而淋巴结微转移是其中最为