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脓毒症急性肾损伤早期诊断及生大黄疗效研究
脓毒症急性肾损伤早期诊断及生大黄疗效研究
[摘要] 目的 探讨脓毒症急性肾损伤的早期诊断指标及生大黄对其的保护作用。 方法 将60只大鼠按随机数字表法分为模型组(A)、治疗组(B)和对照组(C)三组,每组20只。模型组腹膜内注射脂多糖5 mg/kg诱导建立AKI模型,治疗组在腹膜内注射脂多糖即刻胃管内注入生大黄粉溶液,对照组腹膜内注射等量生理盐水。三组大鼠分别在处理前即刻(基础值,0 h),处理后1、6、24 h测定尿Netrin-1及尿KIM-1含量,同时采集血样测定肌酐含量。 结果 大鼠注射脂多糖6 h后,血清肌酐及尿KIM-1水平开始明显上升,注射后1 h尿Netrin-1即有明显上升,6 h达到峰值,24 h左右逐渐下降;生大黄治疗组大鼠各时间点血清肌酐、尿Netrin-1及尿KIM-1上升程度均较模型组明显降低(P0.05)。 结论 尿Netrin-1对脓毒症急性肾损伤有早期诊断作用,生大黄可降低脓毒症急性肾损伤大鼠Scr、尿Netrin-1及尿KIM-1水平,对肾脏有保护作用。
[关键词] 急性肾损伤;脓毒症;生大黄;Netrin-1
[中图分类号] R692 [文献标识码] A [文章编号] 1673-9701(2015)16-0004-04
[Abstract] Objective To study early diagnostic index of septic acute kidney injury(AKI) and protective effect of rhubarb on septic AKI. Methods Sixty rats were randomly divided into the following 3 groups: model group (A), treatment group (B) and control group (C). Each group contains 20 rats. Rats from group A were received a intraperitoneal injection of lipopolysaccharide (LPS) at a dose of 5 mg/kg to induce AKI. Rats from group B were given rhubarb powder solution by oral administration immediately after LPS injection. Rats from group C were treated with the same volume of normal saline (NS) by intraperitoneal injection. Both serum level of creatinine (Scr) and urinary levels of Netrin-1 and KIM-1 of rats in 3 groups were detected in the different time points of treatment (0 h, 1 h, 6 h and 24 h). Results The levels of KIM-1 and CR in rats treated with LPS were obviously increased at the time point of 6 h (P0.05). The urinary level of Netrin-1 began to increase at the time point of 1 h and reached the highest level at the time point of 6 h, then gradually decreased at the time point of 24 h after the injection of LPS. In group B, the levels of Scr, Netrin-1 and KIM-1 were significantly lower as compared with group A (P0.05). Conclusion The urinary level of Netrin-1 might has early diagnostic value in septic acute kidney injury. Rhubarb could protect the kidney of AKI model rats by decreasing the serum level of Scr, urinary
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