胆囊癌中骨桥蛋白和MMP9表达及其意义.docVIP

胆囊癌中骨桥蛋白和MMP9表达及其意义 　　[摘要] 目的 了解胆囊癌组织中骨桥蛋白和MMP-9的表达及其意义。方法 选择正常的胆囊组织10例、胆囊腺瘤组织10例和胆囊癌组织40例，运用免疫组织化学方法，检测OPN和MMP-9的表达水平，对OPN和MMP-9表达的相关性进行分析研究。结果 胆囊癌组织中OPN和MMP-9的表达水平明显高于胆囊组织和胆囊腺瘤组织（P＜0.05），其中在有转移的胆囊癌组织中OPN的表达水平明显高于没有转移的胆囊癌组织（P＜0.05）。胆囊癌组织中OPN和MMP-9的表达具有一致性（P＜0.05）。结论 OPN与MMP-9的表达情况与胆囊癌浸润、转移发生有密切的关系，本实验结果证实MMP-9和OPN均阳性表达时对胆囊癌转移及预后有重要的预测价值。 　　[关键词] 胆囊癌；肿瘤转移；骨桥蛋白；MMP-9；免疫组化 　　[中图分类号] R735.8 [文献标识码] B [文章编号] 1673-9701（2011）31-61-02 　　 　　Expression of Osteopontin（OPN）and MMP-9 in Gallbladder Cancer and Its Clinical Significance 　　XU Guixiang1 WANG Zhenxu1 XU Zhun2 HU Jingjin1 WANG Lin1 TAO Ketao1 HE Songxiong1 ZHANG Guixin1 AN Xin3 XUE Bingchuan3 　　1.Department of General Surgery，Qingdao Fuwai Cardiovadcular Hospital，Qingdao 266034，China；2.Qingdao Foreign Language College，Qingdao 266000，China；3.Department of General Surgery，the Third Affiliated Hospital of Shanxi Medical University， Taiyuan 030053，China 　　 　　[Abstract] Objective To explore expression of osteopontin and MMP-9 in gallbladder cancer and its clinical significance. Methods The expression of osteopontin and MMP-9 was detected by immunohistochemical method in 40 cases of gallbladder cancer，10 cases of normal gallbladder and 10 cases of gallbladder adenoma. The relationship between the expression of osteopontin and MMP-9 was analyzed. Results The positive expression rates of osteopontin and MMP-9 in gallbladder cancer tissues were significantly higher than that in normal gallbladder tissues and gallbladder adenoma tissues（P＜0.05）. The positive expression rates of osteopontin in patients of NEVIN Ⅳ，Ⅴstages were higher than that in patients of NEVIN Ⅰ，Ⅱ，Ⅲ stages）（P＜0.05）. The expression of osteopontin was positively correlated with the expression of MMP-9（P＜0.05）. Conclusion Both osteopontin and MMP-9 are significantly related to the metastasis of gallbladder cancer，and overexpression of both osteopontin and MMP-9 has a predictive value for metastasis and recurrence. 　　[Key words] Gallbladder cancer；Tum