镧对铁皮石斛幼苗生理特性及品质影响.docVIP

镧对铁皮石斛幼苗生理特性及品质影响 　　摘要：为了研究稀土镧（La3+）对铁皮石斛（Dendrobium officinale Kimura et Migo）幼苗生理特性和品质的影响，在温室里用5～120 mg/L的镧溶液分别对铁皮石斛幼苗进行了喷施试验。结果显示，随着镧溶液浓度的升高，幼苗叶片和茎秆中叶绿素、可溶性蛋白、脯氨酸、多糖、石斛碱的含量呈先增加、后降低的变化趋势，而丙二醛的含量先降低、后增加。综合而言，用20 mg/L镧溶液浓度喷施铁皮石斛幼苗为最佳处理浓度，此时叶片中叶绿素的含量比空白对照增加54.37%、可溶性蛋白含量增加53.64%、脯氨酸含量增加99.20 %、丙二醛含量降低44.71%、多糖含量增加56.60%、石斛碱含量增加14.06%；茎秆中叶绿素的含量比空白对照增加17.48%、可溶性蛋白含量增加56.57%、脯氨酸含量增加253.64%、丙二醛含量降低74.91%、多糖含量增加88.73%、石斛碱含量增加24.54%。表明20 mg/L的镧溶液浓度处理能有效的促进铁皮石斛的光合作用、增强抗逆性和提高品质。 　　关键词：镧；铁皮石斛（Dendrobium officinale Kimura et Migo）；生理指标；品质 　　中图分类号：O614..33+1；S567.23+9；Q945 文献标识码：A 文章编号：0439-8114（2015）12-2935-04 　　DOI：10.14088/j.cnki.issn0439-8114.2015.12.032 　　Effect of La3+ on Physiological Characteristics and Quality of 　　Dendrobium officinale Seedlings 　　XU Xiao-yan， TANG Di， CAI Jing-wen， ZHU Hui-fei， SHI Jun 　　（Jiangsu Polytechnic College of Agriculture Forestry， Jurong 212400， Jiangsu， China） 　　Abstract： In order to study the effect of different concentration of Lanthanum （La3+） on some physiological characteristics and quality of Dendrobium officinale Kimura et Migo seedlings， the seedlings was sprayed by 5 to 120 mg/L La3+ in the greenhouse. The results showed that the content of chlorophyll， proline， soluble protein， polysaccharide and dendrobine in leaves and stems increased and then decreased with the increasing of La3+ concentration； while the content of malondialdehyde decreased before it increased. Combined with all the indexes， 20 mg/L La3+ solution was the best treatment concentration as the content of chlorophyll increased by 54.37%； soluble protein increased by 53.64%， proline increased by 99.20%， malondialdehyde decreased by 44.71%， polysaccharide increased by 56.60% and dendrobine increased by 14.06% in leaves compared with the control. The content of chlorophyll increased by 17.48%， soluble protein increased by 56.57%， proline increased by 253.64%， malondialdehyde decreased by 74.91%， polysaccharide increased by 88.73% and dendrobine increased by