阿托伐他汀对血管平滑肌细胞中转染TNFα启动子活性影响研究.docVIP

阿托伐他汀对血管平滑肌细胞中转染TNFα启动子活性影响研究 　　基金项目本课题受国家自然科学基金（NoNo，江苏省高校自然科学基金（No.08KJD320013）和徐州医学院院长专项人才基金（No.07KJZ26）资助 　　doi：10.3969/j.issn.1007-614x.2014.22.2 　　摘要目的：探讨阿托伐他汀（Atv）在转录水平的抗炎作用机制。方法：将构建好的含有人类TNF-α启动子的以虫荧光素酶（Luciferase）为报告基因的质粒DNA转染至体外培养的平滑肌细胞中，以AngⅡ刺激并经不同浓度的Atv处理后，通过检测Luciferase的活性来观察启动子活性的变化。结果：启动子在VSMCs中可高效稳定的表达；而经AngⅡ刺激的VSMCs中，TNF-α启动子活性较未受刺激时明显上调（P0.05）。在VSMCs中0.1～10μmol/L的Atv可抑制经AngⅡ刺激后上调表达的TNF-α启动子活性，并且呈现剂量依赖关系（P0.05）。结论：Atv对TNF-α启动子活性的抑制，证明了其对促炎细胞因子在转录水平的潜在抑制作用。 　　关键词阿托伐他汀；血管平滑肌细胞；肿瘤坏死因子；炎性反应 　　Study of the effect of atorvastatin on the transfection of TNF-αpromoter activity in vascular smooth muscle cell 　　Cheng Ying1，Lu Yongzhi2，Sun Min1，Zhang Zhuoqi1 　　Department of Cardiology，Affiliated Hospital of Xuzhou Medical College（Xuzhou City，Jiangsu），2210021 　　The Second Department of Oncology，the Municipal Hospital of Qingdao City，Shandong 2660112 　　Fundation itemThis project was supported by the national natural science foundation of China（No No， the college natural science foundation of Jiangsu Province（No.08KJD320013） and dean special talent fund（No.07KJZ26） 　　AbstractObjective：To explore the anti-inflammatory action mechanism on transcriptional level of atorvastatin（Atv）.Methods： The plasmid DNA had luciferase as the reporter gene and human TNF alpha promoter.The plasmid DNA was transfected to smooth muscle cell in vitro culture.It was stimulated by AngⅡand disposed by different concentrations of Atv.The change of the promoter activity was observed by detecting the activity of Luciferase.Results：The promoter could highly efficient and stable expression in VSMCs.While VSMCs was stimulated by AngⅡ，TNF-αpromoter activity was obviously up-regulated than without stimulation（P0.05）.0.1～10μmol/L Atv could inhibit the higher expression of TNF-αpromoter activity in VSMCs，and it was a dose-dependent manner（P0.05）.Conclusion：The inhibition of Atv on the TNF-αpromoter activity proves its potential inhibition of proin