隐丹参酮对乳腺癌MDA―MB―231细胞迁移侵袭影响及其机制.docVIP

隐丹参酮对乳腺癌MDA―MB―231细胞迁移侵袭影响及其机制 　　[摘要] 目的 探讨隐丹参酮对乳腺癌MDA-MB-231?胞迁移、侵袭的影响及其机制。 方法 用不同浓度的隐丹参酮处理 MDA-MB-231 细胞24 h。采用 MTT、划痕实验和Transwell侵袭实验测定不同浓度隐丹参酮对MDA-MB-231细胞活性、迁移和侵袭的影响。采用Western blot检测MDA-MB-231细胞中c-Src、p-c-Src Tyr416、FAK、p-FAK Tyr576/577、MMP2蛋白表达水平。 结果 与0 μmol/L对照组相比，5 μmol/L、10 μmol/L、20 μmol/L、40 μmol/L隐丹参酮组对 MDA-MB-231 细胞存活率显著降低，且呈剂量依赖（P0.05）。与对照组相比，5 μmol/L、10 μmol/L、20 μmol/L隐丹参酮组对MDA-MB-231 细胞迁移、侵袭率均显著降低，且呈剂量依赖（P0.05）。Western blot结果显示，与对照组相比，不同浓度组经隐丹参酮处理的 MDA-MB-231 细胞，MMP2蛋白水平和 c-Src、FAK蛋白的磷酸化水平均显著下调（P0.05）。 结论 隐丹参酮可抑制三阴乳腺癌MDA-MB-231 细胞活性、迁移和侵袭，其机制可能与其下调c-Src/FAK通路和MMP2表达有关。 　　[关键词] 隐丹参酮；乳腺癌；MDA-MB-231细胞；迁移；侵袭 　　[中图分类号] R273 [文献标识码] A [文章编号] 1673-9701（2017）35-0016-05 　　[Abstract] Objective To investigate the effects and molecular mechanism of Cryptotanshinone on migration and invasion of breast cancer MDA-MB-231 cells. Methods MDA-MB-231 cells were treated with different concentrations of Cryptotanshinone for 24 h. The effects of Cryptotanshinone on cell viability， migration and invasion of breast cancer MDA-MB-231 cells were assessed by MTT assay， wound healing assay and Transwell invasion assay. The protein levels of c-Src， p-c-Src Tyr416， FAK， p-FAK Tyr576/577， MMP2 were detected by Western blot. Results The results showed that 5 μmol/L， 10 μmol/L， 20 μmol/L， 40 μmol/L Cryptotanshinone groups significantly reduced the viability ability of MDA-MB-231 cells in a dose-dependent manner， compared with the control group（P0.05）. 5 μmol/L， 10 μmol/L， 20 μmol/L Cryptotanshinone groups significantly inhibited the the abilities of migration and invasion of MDA-MB-231 cells in a dose-dependent manner， compared with the control group（P0.05）. The result of Western blot showed that Cryptotanshinone inhibited the expression of MMP2 and the phosphorylation of c-Src、FAK in a dose-dependent manner in MDA-MB-231 cells（P0.05）. Conclusion These results suggest that Cryptotanshinone may suppress the viability， migration and invasion of breast cancer MDA-MB-231 cells by