颊针对类风湿性关节炎家兔镇痛效应及脑脊液八肽胆囊收缩素和β―内啡肽影响.docVIP

颊针对类风湿性关节炎家兔镇痛效应及脑脊液八肽胆囊收缩素和β―内啡肽影响 　　摘要：目的 观察颊针对类风湿性关节炎家兔的镇痛效应，并探讨其中枢作用机制。方法 48只青紫蓝兔随机分为正常组、模型组、体针组和颊针组，将后2组又随机分为针后即时（0 h）和针后1 h亚组，每组8只。采用卵蛋白诱导造成类风湿性关节炎模型。体针组针刺双侧“膝眼”和“足三里”1次，颊针组针刺双侧颊针“膝”1次。针刺后比较正常组、模型组、体针组和颊针组家兔关节局部痛阈及脑脊液中八肽胆囊收缩素（CCK-8）和β-内啡肽（β-EP）的含量。以K+导入法引起家兔腿收缩的最小电流强度作为痛阈；用放射免疫法测定β-EP、CCK-8的含量。结果 模型组痛阈明显低于正常组（P0.05）。针刺后2组β-EP含量均升高，颊针组0 h β-EP含量明显高于体针组0 h β-EP含量（P0.05）；针刺后2组CCK-8含量均向正常水平恢复，颊针组0 h CCK-8含量明显高于体针组0 h CCK-8含量（P0.05），颊针组1 h CCK-8含量与体针针组1 h CCK-8含量无明显差异。结论 颊针即时镇痛效应优于体针，针刺促使脑脊液中β-EP含量升高和CCK-8含量向正常水平恢复，这可能是颊针镇痛中枢作用机制之一。 　　关键词：类风湿性关节炎；颊针；针刺镇痛；八肽胆囊收缩素；β-内啡肽；兔 　　DOI：10.3969/j.issn.1005-5304.2012.12.014 　　中图分类号：R245 文献标识码：A 文章编号：1005-5304（2012）12-0037-03 　　Analgesic Effect of Buccal Acupuncture and Changes of β-EP and CCK-8 in Cerebrospinal Fluid of Experimental Rheumatoid Arthritis Rabbits REN Chao-zhan， DU Xiao-zheng， FANG Xiao-li （Gansu College of Traditional Chinese Medicine， Lanzhou 730000， China） 　　Abstract：Objective To observe the analgesic effect of buccal acupuncture and explore its central analgesic mechanism in experimental rheumatoid arthritis rabbits. Methods A total of 48 rabbits were randomly divided into normal control group， model group， body-acupuncture group， buccal acupuncture group， and the later 2 groups were further randomized into 0 h and 1 h subgroups， with 8 cases in each group. The rheumatoid arthritis model was established by egg-albumin. For rabbits of body-acupuncture group， bilateral “Xiyan” （EX-LE5） and “Zusanli” （ST 36） were punctured for 1 min， with the needle retained for 30 min. For rabbits of buccal acupuncture group， acupuncture was applied to “xi” for 1 min， with the needle retained for 30 min. The pain threshold was detected with K+ import stimulation method， and β-EP and CCK-8 contents in cerebrospinal fluid were assayed with radioimmunoassay. Results Compared with the control group， the pain threshold of model group lowered significantly （P 　　Key words：rheumatoid arthritis；buccal acupuncture；acupuncture ana