食管鳞癌中CD44v6和Ecadherin表达及其意义.docVIP

食管鳞癌中CD44v6和Ecadherin表达及其意义 　　摘要目的：探讨CD44v6和E-cadherin在食管鳞癌中的表达及其意义。方法：应用免疫组织化学S-P法检测15例正常食管鳞状上皮组织、20例癌旁不典型增生组织、60例食管鳞癌组织中CD44v6和E-cadherin的表达。结果：在正常食管鳞状上皮组织、癌旁不典型增生组织和食管癌组织中，CD44v6阳性表达率分别为13.3%(2/15)、45.0%(9/20)和83.3%(50/60)，三者间有非常显著性差异(P0.05）。食管癌中CD44v6表达与E-cadherin表达呈负相关(r＝-0.388，P=0.0020.01)。结论：CD44v6和E-cadherin的表达具有负调节的协同作用。CD44v6和E-cadherin的表达与食管癌发生发展、浸润转移和预后密切相关，可作为预测食管癌侵袭转移和估计预后的重要参考指标。 　　关键词食管肿瘤；免疫组织化学；CD44v6；E-cadherin 　　中图分类号 R735.1 文献标识码 A 　　 　　ABSTRACTPurpose：To investigate the clinical significance of CD44v6 and E-cadherin in esophageal squamous cell carcinoma. Methods：Expression of CD44v6 and E-cadherin in 15 cases of normal esophageal mucosa, 20 cases of peritumorous atypical hyperplastic tissues and 60 cases of esophageal squamous cell carcinoma were determined by S-P immunohistochemical technique . Results：In normal esophageal mucosa, peritumorous atypical hyperplastic tissues and esophageal squamous cell carcinoma, the positive rate of CD44v6 was 13.3%(2/15)、45.0%(9/20) and 83.3%(50/60) respectively, there was significant difference between the three groups(P0.05). And it was positively associated with the expression of CD44v6 and negatively with that of E-cadherin in esophageal carcinoma (r＝-0.388，P=0.0020.01). Conclusion：These date show a correlation between the CD44v6 and E-cadherin expression .The expression of CD44v6 and E-cadherin correlate with the development, invasion, metastasis and prognosis in eaophageal squamous cell carcinoma. CD44v6 and E-cadherin can be regarded as important signs of invasion, metastasis and prognosis. 　　KEY WORDSesophageal carcinoma；immunohistochemistry；CD44v6；E-cadherin 　　 　　侵袭和转移是恶性肿瘤最重要的生物学特性。目前，大量的研究证实恶性肿瘤的发生、发展和转移涉及到多基因、多因素及多分子水平阶段过程。粘附分子家族在恶性肿瘤侵袭转移过程中起到至关重要作用。本研究采用免疫组织化学S-P法，对??管癌及其癌旁不典型增生组织和正常食管鳞状上皮组织中CD44v6和上皮钙粘附素(E-cadherin)的表达进行同步检测，旨在探讨它们与食管癌生物学行为的关系及在转移过程中的变化。 　　1材料与方法 　　1.1一般资料 　　选取安徽省立医院胸外科2004年1月～2004年6月手术切除食管癌标本60例。所有病例术前均未行放、化疗，临床资料完整。正常食管鳞状上皮组织15例，选自经病理证实无异常的食管癌手术切缘，癌旁组织20例，选距癌灶2cm以内，