Ang―1与Tie2在高血压患者内皮祖细胞中表达水平及临床意义研究.docVIP

Ang―1与Tie2在高血压患者内皮祖细胞中表达水平及临床意义研究 　　[摘要] 目的 探讨Ang-1及其受体Tie2在高血压患者循环内皮祖细胞中的表达水平并评价其临床意义。 方法 选择杭州师范大学附属医院2012年1月～2013年6月高血压Ⅰ、Ⅱ级和Ⅲ级患者各15例；正常体检人群30名为对照组。RT-PCR法检测各组外周血中内皮祖细胞内Ang-1、Tie2 mRNA表达水平；Western-blot法测定Tie2酪氨酸磷酸化水平。结论 患者循环内皮祖细胞内Ang-1和Tie2表达随高血压分级的增加逐渐下调，治疗后水平升高，可能与高血压病血管内皮细胞修复的调控机制密切相关。 　　[关键词] 高血压；内皮祖细胞；Ang-1；Tie2 　　[中图分类号] R544.1 [文献标识码] A [文章编号] 1673-7210（2014）04（b）-0004-04 　　[Abstract] Objective To discuss the expression of Ang-1 and Tie2 of peripheral endothelial progenitor cells in patients with hypertension， as well as its clinical significance. Methods 15 patients with gradeⅠ， Ⅱ and grade Ⅲ hypertension respectively were studied in the Affiliated Hospital of Hangzhou Normal University from January 2012 to June 2013. 30 healthy subjects were recruited as normal controls. Expression of Ang-1 and Tie2 mRNA of the peripheral blood endothelial progenitor cells were measured by RT-PCR， Tie2 tyrosine phosphorylation level was measured by Western-blot. Results mRNA levels of Ang-1 and Tie2 of endothelial progenitor cells in patients with grade Ⅲ hypertension were lower than those of the patients with grade Ⅱ， Ⅰ hypertension and control group （P 0.05）， the levels of those in patients with grade Ⅱ hypertension were lower than those of grade Ⅰ hypertension and the control group （P 0.05）. Tie2 tyrosine phosphorylation level of endothelial progenitor cells in patients with grade Ⅲ hypertension was lower than that of patients with grade Ⅱ， gradeⅠ hypertension and control group （P 0.05）， and that of grade Ⅱ hypertension patients was lower than patients with grade Ⅰ hypertension and the control group （P 0.05）. After routine treatment， the expression of Ang-1 and Tie2 mRNA or Tie2 phosphorylation level were higher than those of before treatment （P 0.05）. Conclusion Ang-1 and Tie2 expression of circulating endothelial progenitor cells in patients with hypertension reduced with the increasing of hypertension grade， but after treatment these levels elevated， may ha