鱼油对急性重症胰腺炎大鼠肠黏膜屏障影响.docVIP

鱼油对急性重症胰腺炎大鼠肠黏膜屏障影响 　　[摘要] 目的：探讨大鼠急性重症胰腺炎（SAP）与肠黏膜屏障的关系以及鱼油干预的影响，为临床营养支持提供依据。方法：用3.5%牛磺胆酸钠逆行胰胆管注射法诱导SAP模型。48只成熟SD大鼠造模成功后分为两组，生理盐水组（A组，n=24），鱼油干预组（F组，n=24），分别于造模后给予尾静脉营养支持，与实验后6、12、24 h分别处死各组的其中8只大鼠,观察各自胰腺、小肠病理变化并测定其血清内毒素浓度。结果：F组门静脉血内毒素水平低于A组（P0.05）；F组肠道损伤评分低于A组（P0.05）。结论：添加静脉鱼油营养支持能降低SAP时门静脉血中的内毒素水平、促进肠道黏膜的修复、加强肠黏膜屏障的保护作用。 　　[关键词] 鱼油；肠黏膜屏障；内毒素；急性重症胰腺炎 　　[中图分类号] R-332[文献标识码]A [文章编号]1674-4721（2011）04（a）-０20－０2 　　 　　Protective effect of fish oil on intestinal mucosa barrier in severe acute pancreatitis rats 　　WANG Peng1, WU Hua2* 　　1.Shanxi Medical University, ShanxiProvince, Taiyuan 030001, China; 2.Department of General Surgery, the First Hospital of Shanxi Medical University, ShanxiProvince, Taiyuan 030001, China 　　[Abstract] Objective: To investigate the protective effect of fish oil on intestinal mucosa barrier in severe acute pancreatitis (SAP) rats. Methods: 48 SD rats were divided into 2 groups at random (A,F), 24 in each group, group A was given saline water, group F was given fish oil, established model of intestinal injury after feeding rats 6 hours, then group A was given saline water, group F was given fish oil untill be killed. To measured concentration of endotoxin (ET) in portal vein blood supernatant after 6, 12 and 24 hours. Results: Serum ET concentration in group F was significantly lower than group A (P0.05). The degree of injury of intestinal mucosa was much lower in group F than group A. Conclusion: The fish oil can prevent the intestinal mucosa barrier in SAP. 　　[Key words] Fish oil; Intestinal mucosa barrier; Endotoxin; Severe acute pancreatitis 　　 　　急性重症胰腺炎（severe acute pancreatitis, SAP）在临床上起病凶险，发病率高，死亡率高，引起全身广泛炎症反应，容易导致肠黏膜屏障功能的损伤。鱼油在维持肠黏膜形态、保护肠黏膜屏障、防止细菌易位等方面具有积极的作用。本研究通过对急性重症胰腺炎（SAP）大鼠给予静脉鱼油营养支持，观察其对肠黏膜的保护作用，为临床应用提供依据。 　　1资料与方法 　　1.1动物与试剂 　　健康成年SD大鼠48只，雌雄各半，体重210~240 g。内毒素检测采用鲎试剂偶氮显色法，显色基质鲎试剂盒购自厦门鲎试剂实验厂有限公司。 　　1.2 动物模型的建立 　　按人工胰胆管逆行灌注法复制SAP模型，术前12 h禁食，自由饮水，2%戊巴比妥钠腹腔注射麻醉（40 mg/kg），上腹正中切口入腹，轻柔提出十二指肠和游离胰腺，用微动脉夹夹闭肝门处总胆管使之扩张，通过1