Box―Behnken的设计优化黄精多糖酶法提取工艺.docVIP

Box―Behnken的设计优化黄精多糖酶法提取工艺 　　摘要：采用响应面法，以多糖提取率为评价指标，对酶解温度、酶添加量、酶解时间、料液比4个影响因素进行考察，优化酶法提取黄精多糖的提取工艺，另以Design-Expert 8.05软件对数据进行综合统计分析。结果表明，最优工艺为酶添加量3%、酶解温度54.8 ℃、酶解时间99 min、料液比1∶22.4（g/mL），经验证黄精多糖提取率为11.22%。利用Box-Behnken响应面法优选的黄精多糖酶法提取工艺稳定可行，可用于黄精多糖的提取。 　　?P键词：黄精；多糖；响应面 　　中图分类号：R284.2；TQ461 文献标识码：A 文章编号：0439-8114（2017）16-3121-04 　　DOI：10.14088/j.cnki.issn0439-8114.2017.16.031 　　Optimization of Enzymatic Extraction of Polysaccharide from Ploygonatum by Box-Behnken Design and Response Surface Methodology 　　YANG De1，XUE Shu-jing1，HUANG Lei2，GUO Peng1，GAO Hong1，SHI De-fang1，LI Lu1，CHEN Xiao-chun3 　　（1.Research Institute of Agricultural Products Processing and Nuclear-Agricultural Technology， Wuhan 430064，China； 　　2.College of Food Science and Technology，Huazhong Agricultural University， Wuhan 430070，China； 　　3.Yuan Hui Technology Development Co.，Ltd.， Enshi 445000，Hubei，China） 　　Abstract：To optimize the processing of enzymatic extraction of polysaccharide from Ploygonatum. Box-Behnken response surface methodology was used to optimize different enzyme dosage，reaction temperature and reaction time by using Design-Expert 8.05 software for data analysis and processing. According to Box-Behnken response，the best extraction conditions for the polysaccharide from Ploygonatum were as follows：the amount of enzyme complex was 3%，hydrolysis temperature was 54.8 ℃，and reaction time was 99 min，ratio of solid to liquid 1∶22.4（g/mL）. In accordance with the above process，the polysaccharide yield was 11.22%. Box-Behnken response surface methodology is used to optimize the enzymatic extraction process for the polysaccharide in this study，which is effective，stable and feasible. 　　Key words： ploygonatum； polysaccharide； response surface methodology 　　黄精为百合科植物黄精（Polygonatum sibiricum Redoute）的根茎，其味甘、性平，有补中益气、润心肺、强筋骨的功能[1，2]。研究表明，黄精多糖是黄精主要功能成分之一，具有延长果蝇寿命、改善老龄大鼠老化相关酶的活性及对老龄大鼠明显的促智作用，现代药理学研究表明其活性成分最主要为黄精多糖（Polygonatum Sibiricum Polysaccharides，PSP），其具有抗肿瘤、调节血糖血脂、调节免疫等生理功能[3，4]，因此黄精多糖