多靶点抑制剂联合细胞毒药物治疗恶性胶质瘤疗效的实验分析-外科学专业论文.docxVIP

宁夏医科大学硕士研究生学位论文摘要 宁夏医科大学硕士研究生学位论文 摘要 malignant glioma efficacy of experimental study ABSTRACT Objective Multi-target inhibitor vandetanib combined cytotoxic drugs carmustin(BCNU) on the proliferation and invasion of C6 rat glioma. Methods Subculture c6 rat glioma cells, the impact of drugs on cell viability using the MTT assay, HE staining observed changes in cell morphology.In situ injection of C6 rat glioma model, six days into the vandetanib group (50mg/kg.d), BCNU group (25 mg / kg.d), the combination group (vandetanib+ BCNU) and control groups, the next administration, two weeks after the decapitation method put to death in rat brain, do the tumor tissue sections to calculate the size of the tumor volume, immunohistochemical SP method was used to mark groups of tumor vascular endothelial cells todetect transplanted tumor microvessel density (MVD), to detect the expression of MMP1 protein related to tumor invasiveness. Groups of apoptotic index by TUNEL method. Results Suppression and promote the apoptotic effect on tumor cells, the combination group were significantly higher than the BCNU group (P 0.01). Compared with the control group, the group of BCNU tumor volumes decreased by 34.0% (P 0.05) ，xenografts MMPl expression as (+ +) ， MVD was no significant change ， 62.6% of the rate of apoptosis of transplanted tumor cells (P 0.05); combination group tumor volumes decreased by 56.0% (P 0.05) ，xenografts MMPl expression positive (+) ，MVD was reduced 53% (P 0.05). apoptosis rate of 82.0% (P 0.05); Conclusion The combination therapy can not only significantly inhibit the c6 glioma cell tumor growth but also reduce tumor invasion, and to provide experimental basis for the joint multi-target inhibitors and cytotoxic drugs in clinical treatment of malignant glioma. KEYWORDS C6 rat glioma，multi-target inhibitors， cytotoxic drugs，efficacy studies 符号说明 宁夏医科大学硕士研究生学位论文符号说明 宁夏医科大学硕士研究生学位论文 符号说明 脑肿瘤干细胞（BTSCs） 替莫唑鞍（TMZ） 卡莫司汀（BCNU） 血管内皮细胞生长因子(VEGF) 血管内皮细胞生长因子受体( VEGFR) 金属蛋白