肠外谷氨酰胺补充对脓毒症大鼠淋巴器官细胞凋亡的影响-急诊医学专业论文.docxVIP

Ab Abstract PAGE PAGE IV ABSTRACT Objective： Sepsis is still one of the important causes of death in critically ill patients, the apoptosis of lymphocyte cell is one of the important pathophysiological mechanisms of sepsis. we investigated the effects of the apoptosis of lymphoid organs in sepsis rats which was received different doses of glutamine by parenteral administration . Methods: 、 SD rats were randomly divided into five groups:The sham operation group (SHAM), Model group (CLP), Low doseglutamine group (L-GLN), Medium dose glutamine group (M-GLN), High dose glutamine group (H-GLN) ， established the rat sepsis model by cecal ligation and puncture in the group of CLP、L-GLN 、 M-GLN、H-GLN，rats of every group were respectively received the tail intravenous injection of saline、0.35g/kg glutamine、0.50g/kg glutamine、0.75g/kg glutamine after surgey immediately.At the same time, in order to eliminate the influence of the operation itself, rat tail vein injection of saline was served as sham operation group. 、 Observe and record the performance of rats in every group after surgery, measured respectively rectal temperature, heart rate, breath of rats in every group in abdominal anesthesia after established models for 24 hours, plucked blood by common carotid artery and centrifuged,taked the serum for detection ,at same time ,plucked blood by common carotid artery from three rats of every group randomly for blood culture,and taked their spleen and thymus into 10% formalin fixed, paraffin embedded for immunohistochemistry. 、 The cell apoptosis of spleen and thymus tissue was deteced by TUNEL, Immunohistochemistry was employed for detecting the expression of caspase3 、 HSP90 protein in spleen and thymus tissue ,the serum level of IgA、IgG、IgM and TNF-α、IL-10 in rats was detected by ELISA. Results: 1、Comfirmed the rat sepsis model was successfully established：monitored the PAGE PAGE V rectal temperature, heart rate, breath of rats in every group in abd