二乙基(3-甲基脲)(苯基)甲基磷酸酯(DEP)分子印迹聚合物的制备及其性能评价-物理化学专业论文.docxVIP

Abstract Molecularly-Imprinted Polymers (MIPs) is the material which has the function of remembrance and recognition for template, and it has the merits of HYPERLINK /simple/ simple in HYPERLINK /preparation/ preparation, HYPERLINK /cheaply/ cheaply making, steady in performance and also repeated HYPERLINK /use/ use. Therefore, MIPs can be widely used in detection the organophosphorus pesticides residues. Recently, MIPs mostly be HYPERLINK /synthesize/ synthesized by using the targete molecules as template, and the template HYPERLINK /can/ can happen leak out of the polymer and interferes in the following analysis . In this paper, by synthesis diethyl (3-methylureido) (phenyl) methylphosphonate (DEP), we prapared the MIPs by precipitation polymerization using DEP as dummy template to study it’s specific affinity and also the HYPERLINK /ability/ ability to recognize a series of pesticides, which are the structural analogues of DEP and mostly belong to organophosphorus pesticides . 1、Based on the HYPERLINK /computer/ computer HYPERLINK /simulation/ simulation and by doing series NMR and adsorb experiments , we selected a best imprinting condition: DEP as dummy template, methacrylic acid (MAA) , ethyleneglycol dimethacrylate (EDMA) and chloroform as functional monomer , cross-linker and porogen ， respectively , and the ratios of the DEP:MAA: EDMA =1:9:45. 2、Equilibrium rebinding experiments indicated that the adsorption capacity of MIPs for DEP is two times of control polymer (NIPs) . Scatchard plot of analysis revealed that there was two classes binding sites populated in the imprinted polymer with dissociation constants of 0.5mmol/L for high affinity sites and 26 mmol/L for low affinity sites , an apparent maximum binding constant of 1.31μmol/g for high affinity sites and 2.41μmol/g for low affinity sites. 3、Binding selectivity shows that MIPs exhibited better selectivity and recognizability for methamidophos and orthene which contain