慢性前列腺炎并不育患者血清抗精子抗体定量测定剖析.docVIP

慢性前列腺炎并不育患者血清抗精子抗体定量测定剖析 　　[摘要] 目的：探讨慢性前列腺炎并不育患者血清抗精子抗体与不育的关系，为临床治疗提供依据。方法：采用酶联免疫吸附法（ELISA）检测80例慢性前列腺炎并不育患者（A组）血清抗精子抗体（AsAb），同时选择正常生育的慢性前列腺炎患者50例（B组）作对照。结果：A组80例，检出AsAb阳性37例，阳性率46.3%，抗精子总抗体（T-Ig）（126.25±45.47）U/ml，B组AsAb阳性6例，阳性率12.0%，T-Ig（41.25±14.20）U/ml，两组阳性率及抗精子总抗体比较，差异均有统计学意义（均P0.05）。结论：慢性前列腺炎并不育患者存在免疫性因素，ELISA方法敏感度高，操作简便，对临床合并前列腺炎的不育患者探求病因提供指导。 　　[关键词] 前列腺炎；男性不育症；抗精子抗体；酶联免疫吸附法 　　[中图分类号] R697.33 [文献标识码] Ａ [文章编号] 1673-7210（2011）12（b）-049-02 　　 　　Evaluation of antisperm antibodies detection in infertile men with chronic prostatitis 　　ZHENG Zheming1, YU Zhaocun1*,HUANG Zhihong2, XING Fashu1, CHEN Zaoqing1, HUANG Huasheng1, FANG Zhongjin1, YANG Changjun1 　　1.Department of Urology, Dongguan Houjie Affiliated Hospital, Guangdong Medical College, Guangdong Province, Dongguan 523945, China; 2. Department of Laboratory, Dongguan Houjie Affiliated Hospital, Guangdong Medical College, Guangdong Province, Dongguan 523945, China 　　[Abstract] Objective: To investigate the relationship between antisperm antibody (AsAb) and infertility men with prostatitis, and to provide some clue for clinic. Methods: Antisperm antibodies for infertile men with chronic prostatitis (n=80, group A) and fertile men with chronic prostatitis (n=50, group B) as control were respectively detected by the ELISA method. Results: The antisperm antibody was (126.25±45.47) U/ml in group A and (41.25±14.20) U/ml in group B, and the positive rate was 46.3% and 12.0% respectively, and there were significantly differences between two groups (all P0.05). Conclusion: Infertile men with chronic prostatitis can be relate to sperm autoimmunity, ELISA method is a highly sensitive and easy experiment method, which can explore the cause of unknown infertility and provide the guidance of clinical treatment and prognosis. 　　[Key words] Chronic prostatitis; Male infertility; Anti-sperm antibody (AsAb); Enzyme linked immunosorbent assay (ELISA) 　　慢性前列腺炎是成年男性常见的生殖道感染性疾病，可通过影响精子的数量和质量而导致不育[1-2]，但其机制尚不完全明确。笔者采用酶联免