低频低强度超声波诱导白血病细胞株K562凋亡的研究..docVIP

低频低强度超声波诱导白血病细胞株K562凋亡的研究 ：马燕，陈宝安，吴巍，姜藻，钱梦 ［ ］目的：研究低频低强度超声波诱导白血病细胞 株K562凋亡的作用及其机制。方法：取对数生长期的K562 细胞，分成实验组和对照组，用MTT法计算细胞存活率，流 式细胞仪判断细胞凋亡，瑞氏染色和透射电镜观察细胞照射 后有无出现凋亡，分光光度法检测照射前后K562细胞C aspase3的活性改变。结果：超声照射各组间细胞存活率与 对照组相比有统计学差异，但组间无差异；瑞氏染色光镜下 可见组和组细胞出现细胞核固缩等早期凋亡的改变，组出现 凋亡小体等典型凋亡改变；流式细胞仪检测组凋亡率为％; 照射前后K562细胞Caspase3的活性无差异。结论：低频低 强度超声波对白血病细胞株K562有诱导凋亡作用，与照射 强度和时间无明显相关。低频低强度超声波诱导K562的凋亡 可能不通过Caspas e3的途径。 ［关键词］超声；白血病细胞株K56 2;凋亡 Abstrac t： Objective Toinvestigat etheeffectof lowfrequency andlowintens ityultrasoun donleukemiac elllinesK562andtoclarify theroleofCas pase3intheef feet. Methods K562cellsin logphasewere dividedintoc ontrolgroupa ndexperiment algroup. Thei ntensityoflo wfrequency (2 OkHz)andlowi ntensityultrasoundwere,a nd ? cm-2expos uretimewere3 0and60srespe ctively. K562 cellswereinc ubatedin24we llculturepla tesfordiffer enttimeofper iods(6,24,48h)aftersonic ationandthen umberofvital cellswastest edbyMTTassay . Themorpholo gyofapoptosi swasanalyzed byWright’ sst ainandtransm issionelectr onmicroscopy . Thepercenta geofapoptosi swerestudied byflowcytome try(FCM). The activationof Caspase3wast estedbyCaspa se3kitwithsp ectrophotome tricmethod.R esults Theab sorbancevalu eofMTTdecrea sedsignifica ntlyafterexp osure. Thehig hestapoptosi swasfounding roupafter30s sonicationan d6hincubatio n. Morphologi calalteratio nsobservedin cellsafterex posuretoultr asoundinclud ed：cellshrin kage,membran eblebbing,ch romatinconde nsation,nucl earfragmenta tion,andapop toticbodyfor mat ion. FCMre vealedthatth epercentageo fapoptosisof cellsincontr olgroup,inWg roup,ingroup andingroupwa s%,%,%and%we renosignific antdifferenc eofactivatio nofCaspase3b etweencontro landexperime ntalgroup(gr oupafter30ssonicationand 6hincubation ). Conclusion Lowfrequenc hyandlowinte nsityultraso undcaninduceapoptosisinK 562celllines aftertheeffe ctisnotrelev anttotheinte nsityandexpo suretimeofultrasound. Ke ywords : ultra sound ； malign antmyeloidce 11linesK562； apoptosis 超声是一种机械波，对正常组织损伤小，而其诱导肿瘤 细胞