高产纤维素酶菌株的筛选、鉴定及内切葡聚糖酶高表达基因工程菌的构建-生物学、微生物学专业论文.docxVIP

ABSTRACTCellulose ABSTRACT Cellulose widely distributed the earth，everywhere，our country is humongous， rich in natural resources，and cellulose resources is extremely rich，but，because of its characteristics of structure stability,not easy to hydrolysis，greatly limits its use range．If they produce Can make of large number of microorganisms，degradation of cellulose enzyme，by converting：cellulose decomposing enzyme，can not only solve the problem of environmental pollution，but also reasonable use of fiber resources．In addition，the reasonable use of fiber resources，the development of feed resources，agricultural energy production，the development of animal husbandry resources，reduce the environmental pollution is the human is of great significance． Although people in the screening of cellulose enzyme，done lot of research work,and has obtained certain achievements，but until now,enzyme production ability of high strain due to its own limitations，is not very good use。With the development of biotechnology,cut inside the beta glucan enzyme gene cloning and expression of research more and more deeply,but，because of various conditions such as heat resistance is poor,poor stability,really into the production practice of genetic engineering bacteria few and far between．Therefore，the method of using reasonable cut inside to build high beta glucan enzyme gene engineering bacteria and applied to the production practice，will greatly speed up the pace of fibrous raw materials in the use of industrial and agricultural production． 1．This experiment adopts the Congo red staining method(Congo red staining)，choose III 万方数据 万方数据 万方数据 the the brush，rotten wood and straw accumulation is rich in cellulose content in the soil sampling， through the solid medium carboxymethyl cellulose sodium concentration after separation of single colony,with Congo red staining method(Congo red staining)to sieve，sieve out 6 strains of hydrolysis circle is big，high strain of CMC enzyme activity,after the number,th