高产纤维素酶真菌的筛选、鉴定及其在畜禽粪便处理中的应用-生物化学与分子生物学专业论文.docxVIP

r7 吖 A bstract 舢Y iii“●■- 删8 ㈣0 M6 舢5 删9跚9 Abstract The author of this article separates 3 5 strains of fungi which can break down cellulose better from the forest humus soil and pig manure composting。Through screening of plate firstly and Re-screening of fermentation secondly,we get 5 strains of fungi which have the strong ability of enzyme production，named respectively：M一5，M一7， M-8，M-1 1，M-16，Ofcourse，M一5 is the most ofall． Through the Identification of morphology and ITS conserved sequences of rDNA， M一5 strain is identified as Aspergillus clavatonanicus． Research the solid—state fermentation conditions on M一5 strain，we can get the optimal conditions：carbon source are rice husk：bran=7：3，and the rice husk is pretreated by 3％NaOH；Nitrogen source are 1％州H)2S04；Zhe ratio of solid to liquid 1：2．5； Inoculation volume of 1 05 spores／(g dry fermented matter)l ml；PH 6．0，Cultured 84h around 30C．Under the optimal conditions，CMC activity and FPA are 3015．4 umol／g??h and 432．9umol／g．h respectively． Studies on the character of CMC enzyme and filter paper enzyme of M-5 strain，we get the following results：optimal PH and temperature of CMCase are PH 8．0 and 50C， filter paper enzyme PH5．0，50。C；We also study the effect of several metal ions on the enzyme activity；Finally,we study the stability of PH and temperature of CMC enzyme and filter paper enzyme respectiVely，they are both acidic and megatemperature enzymes，they both can withstand relatively hi曲temperatures． M一5 strain as the main strain，other strains as bacteria—assisted，we study the role of microbial treatment of livestock and poultry manure for resource．The results shows that the inoculation of exogenous microbial preparation will help shorten the composting time， improve product quality composted；Afier the comparison of effect of composting among several microbial designs，It is found that design 3 has the best effect of composting； Compared to commonly used microbial preparation on the market，the effect of comp