高产虾青素的红发夫酵母菌株进化工程研究-生物化工专业论文.docxVIP

ABSTRACT Astaxanthin is a kind of carotenoid ，which can high幽efficiently quenching active oxygen，thus avoiding the destruction of organisms by active oxygen. It is a strong antioxidant ，broad used in food，pharmaceutical ，叫uaculture and 50 on. Phaffia rhodozyma is a yeast that can produce astaxanthin highly. It has some advantages such as shorter period of fermentation ，easy to cuhure in a high density，and so on. In this 地也 paper，an evolutionary engineering method was used for breeding Phaffia rhodozyma ‘ high-yield strains. The wild strain Phaffia rhodozyma As 2.1557，as the starting strain，was treated with UV andN丁G，and Genetic diversity of strains library was got.In the shake t1ask， 萨 ionone，DPA，NaCI，Ti02，日202，and NaCIO was used as evolutionary pressures for evolutionary engineering. The transit time was 48h. The evolutionary engineering studies have found that these substances could inhibit the growth of Phaj，罚。 胁。dozyma，thus high biomass strains was obtained when the pressure was disinherited. 丁he impact of carotenoids synthetic inhibitors such as βionone and DPA on the accumulation of astaxanthin is not obvious. But NaCl，Ti02，H202，and NaCIO which can produce active oxygen， are able to stimulate the accumulation of astaxanthin. We screened on the evolutionary yeasts，and got s?X astaxanthin. overproducing strains. Their physiological characteristics were studied. The results showed that evolution greatly changes the Biological traits.ηle yeast color of liquid culture changes 企om orange to pink，and the biomass increased more than twice. Compared with the wild type，the colony of plate culture has changed greatl弘 Its diameter 由‘ became larger，and its color changes 仕om orange to pink. different 仕om the wild-types flat，rough and dry colony，the colony evolved into convex， wet， 也 suggesting that strains produced capsules. Microscopic observations on the evolutio