高分子肝支架材料半乳糖化壳聚糖的制备和研究-生物医学工程专业论文.docxVIP

武汉理工大学硕士学位论文Abstract 武汉理工大学硕士学位论文 Abstract Liver is one of the most important and complex organs，serving several indispensable functions such as metabolism，elimination of toxicant，regulation of circulation in the body．Currently，many patients with liver failure are suffering from loss of liver functions．So the research of optimal liver scaffold became the urgent task of medicine and biological material fields。Chitosan as n binary heteropolysaccharide containing linked 2-acetamide·-2·-13-D·-glucopyranose and 2-amino··2·deoxy·-13一D—glucopyranose residues has characteristic similar to glycosaminoglycans and is biodegradable and non-toxic．The chitosan molecule has amino and hydroxyl groups which Call be modified chemically．Hepatocytes are anchorage—dependent cells on which surfaces asialoglycoprotein receptors exist， however galactose is known as a specific adhesive ligand to asialoglycoprotein receptor(ASGR、of hepatocyte．The introduce of the galactose residues could strengthen the anchorage site within the scaffold and promote the specific cell interactions and various cell fuctions including migration，proliferation，and specific gene expression．Hepatocyte in vivo grows in the three dimensions and cross—link network composed of various ECMs and in vitro this anchorage—dependent cells must adhem to materials to grow and metabolism．So from the point of view of improving chemical structure of materials and geometry size，we mimic the vivo three—dimension environment and prepare the porous galactosylated chitosan scaffold to culture hepatocyte fur promoting the culture density and the metabolic activation． In this paper，the porous galactosylated chitosan was synthesized by the reaction the amino group of chitosan and of carboxyl group of lactobionic acid under activiation of EDC and NHS；freeze—drying technique．The structure and physical behaviors were characterized by FT-tR，1HNMR DS c，XRD,water contact angle and the morphology and properities were analysed．The results co