自制肝保护液对梗阻性黄疸大鼠门静脉阻断所致肝细胞损伤的保护作用.docVIP

自制肝保护液对梗阻性黄疸大鼠门静脉阻断所致肝细胞损伤的保护作用 冯春林1，2，林 恒1，陈 炜2，冷 凯2，郑晓华2，主鹤亭2，别 平1 （400038 重庆，第三军医大学西南医院肝胆外科研究所1；563000 贵州 遵义，遵义医学院第三附属医院肝胆外科2） [摘要] 目的 探讨自制肝保护液对梗阻性黄疸大鼠门静脉阻断所致肝细胞损伤的作用及对肝细胞MAPKs信号途径的影响。方法 72只Wistar系大鼠完全随机分为4组：A组：胆道再通、门静脉周围游离、切除肝尾状叶，90 min后关腹腔；B组：胆道再通、肝尾状叶分流门静脉、余肝门静脉血流阻断90 min、恢复正常门静脉血流、切除肝尾状叶，关腹；C组：B组＋门静脉肝侧灌注4 ℃乳酸林格氏液；D组：B组＋门静脉肝侧灌注4 ℃自制肝保护液（home-made liver solution，HMLS）。术后0、6、24 h获取各组大鼠组织标本。采用RT-PCR和Western blot分别测定肝组织A20 mRNA和蛋白表达，测定肝组织中p-JNK、p-ERK、p-p38、Bcl-2、Bax和Caspase-3的蛋白表达情况，TUNEL法检测肝细胞凋亡指数。结果 D组大鼠A20 mRNA和蛋白表达均明显增加（P0.05），p-JNK、Bax、Caspase-3蛋白表达明显下降（P0.05），而p-ERK、Bcl-2蛋白表达明显增加（P0.05）。C、D组大鼠与B组相比肝细胞凋亡率明显下降（P0.05），其中D组下降更明显。结论 自制肝保护液经门静脉在体持续低温灌注肝脏，可以促进A20 mRNA及其蛋白的高表达，由此激活ERK信号转导通路，抑制JNK信号转导通路，反向调节线粒体途径Bcl-2/ [关键词] 梗阻性黄疸；门静脉血流阻断；自制肝保护液；MAPK信号转录途径 [中图法分类号] [文献标志码] A Effect of homeself-made liver solution on MRPK signal transcriptional pathway in obstructive jaundice rats after portal vein occlusion in rats with obstructive jaundice Feng Chunlin1，2, Lin Heng1, Chen Wei2,Leng Kai2,Zheng Xiaohua2, Zhu Heting2, Bie Ping1 (Institute of Hepatobiliary Surgery,Southwest Hospital,Third Military Medical University,Chongqing, 400038;Department of Hepatobiliary Surgery, Third Affiliated Hospital of Zunyi Medical College,Guizhou, Zunyi Province, 563000,China)Feng Chunlin1, 2, Lin Heng1, Chen Wei2, Leng Kai2, Zheng Xiaohua2, Zhu Heting2, Bie Ping1 (Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038; Department of Hepatobiliary Surgery, Zunyi Hospital, Zunyi, Guizhou Province, 563000, China) [Abstract] Objective To explore the effect of homeself-made liver solution on MRPK signal transcriptional pathway after portal vein occlusion in rats with obstructive jaundice. Methods 72Seventy-two Wisitar rats were randomly divided into four groups:, group A (biliary tract recanalization, liberation of portal vein, resection of caudate lobe of liver and closure of abdomen ati