地塞米松和地塞米松透明质酸圆窗灌注对豚鼠圆窗膜形态学的影响研究-耳鼻咽喉科专业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 结论：光镜和扫描电镜下观察：(1)圆窗放置吸收性明胶海绵 对豚鼠圆窗膜形态无明显影响。(2)地塞米松和地塞米松一透明质酸 圆窗灌注对豚鼠圆窗膜的形态无明显影响。 关键词： 地塞米松，透明质酸，吸收性明胶海绵，圆窗膜，形 态学 Ⅱ 硕士学位论文 硕士学位论文 英文摘要 Abstract Objective：The goal of this study was to observe the morphologic changes of round window membrane in guinea pigs after place Gelfoam in round window niche，intratympanic perfusion dexamethasone and dexamethasone／hyaluronic acid． Methods：Thirty·six healthy male guinea pigs were used in the sl：Ildy．these animals were divided randomly into three groups(each had 12 animals)．In Group I，Gelfoam Was placed in round window niche and a pipe Was introduced onto the round window．In Group II，Gelfoam soaked with hyaluronic acid was placed in round window niche，and a pipe Was introduced onto the round window，dexamethasone Was perfused through the pipe once a day for 7 days．In Group III，Gelfoam Was placed in round window niche，a pipe Was introduced onto the round window，and dexamethasone Was perfused once a day for 7 days．In Group IV was normal control group(n--6)．The animals were sacrificed and the temporal bones、Ⅳerc harvested at lday or／days after finished dexamethasone perfusion or operative procedure．The morphologic changes of round window membrane were studied by both light microscope and scanning electron microscope．The thickness of round window membrane was measured and compared with that of normal control group． m 硕士学位论文 硕士学位论文 英文摘要 Results：(1)All animals tolerated the procedure well，no one death or abnormal behavior．(2)No inflammatory reaction and bleeding was noticed in middle ear cavity．(3)Morphologic changes observed by light microscope：the three layers of round window membrane including an outer epithelial layer facing the middle ear cavity。the middle cOre of connectiv