乌贼墨多糖联合顺铂对乳腺癌细胞凋亡与自噬的作用机制研究水产品加工及贮藏工程专业论文.docxVIP

导凋亡现象的发生。利用MDC染色观察药物对MDA．MB．23 导凋亡现象的发生。 利用MDC染色观察药物对MDA．MB．23 1细胞自噬的影响，并根据Western blot 结果分析自噬相关蛋白与P13K-Akt和p38MAPK信号通路的关键调控因子的表达情 况，还初步讨论药物作用下MDA．MB．23 1细胞发生凋亡与自噬的相互影响及分子机 理。由MDC染色结果分析，DDP能够诱导MDA．MB．231细胞自噬，但SIP+DDP 组自噬率明显低于DDP组，且两组之问差异显著(尸0．05)，由此推断，SIP可一 定程度缓解DDP引起的肿瘤细胞自噬。western blot结果分析，与DDP组相比， 3-MA+DDP组和SIP+DDP组中，LC3 I向LC3 II的转变程度均降低，LC3 II蛋白表 达水平也极显著下降(尸0．01)，而Beclin．1蛋白表达水平均也表现为极显著(尸0．01) 降低现象。结合凋亡相关蛋白表达与P13K．Akt和p38MAPK信号通路的关键调控因 子p-Akt和p-p38的表达情况来看，DDP能诱导肿瘤细胞发生凋亡和自噬，且可从 P13K．Akt和p38MAPK途径调控，前者可诱导自噬拮抗凋亡，后者自噬和凋亡均可得 到促进。SIP可抑制P13K．Akt以及p38MAPK相关途径，拮抗自噬，并促进细胞凋亡 现象进～步发生，从而达到杀死肿瘤细胞的目的。 关键词：顺铂，乌贼墨多糖，乳腺癌细胞，转移，凋亡，自噬 II 万方数据 Study Study on the mechanism of DDP combined with SIP on apoptosis and autophagy in MDA--MB--23 1 Abstract Cisplatin(Cis-dischlorodiammine-platinrn DDP)，a kind of platinum metal complexes，has lethal effect on most of tumor cells，which was the common clinical chemical drugs，but had certain toxic effect when the concentration was too high．SIP was a kind of polysaccharide separated and purified from s．esculenta(Sepia esculenta Hoyle)Monei with high activity and novel structure．Pharmacological activity study showed that SIP had high immune enhancement，anti—oxidation and anti—tumor activity,which Can exert its anti-tumor effect through regulating expression of apoptosis related protein to induce the apoptosis of tumor cells．In order to improve the anti-tumor effect without increasing toxicity and reduce the tumor drug resistance at the same time，multiple anti—cancer drugs were combined to play a synergistic effect between drugs．Carcinoma of the breast is the highest incidence among malignant tumor disease in the world．About 1．2 million women suffer from breast cancer and O．5million died yearly,in addition，the number is still rising．The significant cause of breast cancer to difficult to cure completely was cancer metastasis．Many patients did not died of primary cancer,but metastatic cancer． MDA-．MB．-23 1 cell Was used as DDP chemother