厄贝沙坦对高糖诱导的小鼠足细胞trpc6表达的影响内科学专业论文.docxVIP

实现的。 实现的。 关键词：高糖；足细胞；TRPC6：厄贝沙坦 万方数据 The The effect of Irbesartan on high glucose induced mouse Podocyte TRPC6 expression Abstract： Objective：Diabetic kidney disease is one of the major microvascular complications of diabetes mellitus，Micro—albuminuria is considered to the main clinical manifestations in early of disease。In recent years，abundant researches show US that podocyte injury is one of the main causes of proteinuria and become a hot research topic．Transient receptor potential cation channel 6 is one of the major components of the podocyte slit diaphragm proteins，which expression abnormalities can lead to the occurrence of urinary protein．In this experiment，we research the effect of expression irbesartan(angiotensinlI receptor blockers)on high glucose induced the of TRPC6 in conditional immortalized mouse podocyte，and investigate the possible mechanism of irbesartan on protective podocyte in diabetic kidney disease．Method：To begin with，the mouse podocyte were divided into five groups：hypertonic control group(mannitol 30mmol／l+glucose 5mmol／1)， normal control group(5mmol／L glucose)，experimental group(Glucose 1 5mmol／L，Glucose 25mmol／L and Glucose 3 5mmol／L)，the morphological changes of the cells induced by different concentrations of glucose were observed under the inverted microscope，Cck-8 was used to detect Cell viability, Immuno—cytochemistry was used to detect changes which the distribution of TRPC6 expression in the podocytes induced by different concentrations of 4 万方数据 glucose，Western glucose，Western blotting was used to detect the expression of TRPC6 protein in the cells of different concentrations of glucose．Reverse transcription —polymerase chain reaction was used to detect the expression of podocyte TRPC6 mRNA in the cells of different concentrations of glucose．Next，hi曲 glucose(25mmol／1)respectively stimulate oh、 1 2h、24h and 48h on podocyte．each time point were collected to extract the total cellular protein and total RNA by WB detection