甘肃贝母组培快繁技术及对其总生物碱积累影响的研究果树学专业论文.docxVIP

甘肃农业大学硕士毕业论文摘 甘肃农业大学硕士毕业论文 摘 要 本文对甘肃贝母的组织培养和生物碱产量进行研究。试验结果如下： 1．诱导甘肃贝母小鳞茎的适合培养基是MS++NAA2mg／L+2，4一D2mg／L+6-BA0．5mg／L+ IBA0．5mg／L+KT0．2mg／L，最适宜温度是20C，最适宜的光照条件是全黑暗。 2．甘肃贝母小鳞茎增殖的适合培养基是MS+NAAl mg／L，最适宜温度是15C，最适 宜的光照条件是全黑暗。 3．甘肃贝母诱导成苗的适合激素是MS+NAA0．2 mg／L+2，4-DO．5 mg／L+6·BAl mg／L， 最适宜温度是20。C，最适宜的光照条件是半光照。 4．甘肃贝母诱导生根的适合激素是MS+NAA0．5 mg／L+IBA0．2 mg／L+VBl mg／L，最适 宜温度是15℃，最适宜的光照条件是全黑暗。 5．甘肃贝母组培再生鳞茎的总生物碱含量高于野生甘肃贝母鳞茎的总生物碱含量。 6．各个培养时期的组培甘肃贝母鳞茎总生物碱含量的对比表明，甘肃贝母鳞茎组织培 养以60天为适宜采收期。 7．暗培养有利于生物碱的生产。 8．甘肃贝母鳞茎在15。C培养下有利于生物碱的生产。 关键词：甘肃贝母 鳞茎 组织培养 快速繁殖 生物碱 甘肃农业大学硕士毕业论文Summary 甘肃农业大学硕士毕业论文 Summary The paper studies on total alkaloid accumulation and the tissue culture of Fritillaria przewalskii F．Przewalskii Maxim．The results are弱follows： 1．by nle suitable hormone for Fritillaria pmewalskii E Przewalskii Maxim induced bulbs is MS++NAA2mg／L+2，4一D2mg／L+6-BA0．5mg／L+IBA0．5mg／L+KT0．2mg／L， the most appropriate temperature is 20℃，the most appropriate light is the darkness． 2．11le suitable hormone for Fritillaria przewalskii E Przewalskii Maxim proliferation of bulb is MS+NAA I mg／L，the most appropriate temperature is 1 5℃，the most appropriate light is the dark． 3．The suitable hormone for Fritillaria przewalskii E Przewalskii Maxim seedling is MS+ NAA0．2 mg／L+2，4-DO．5 mg／L+6-BAl mg／L，the most appropriate temperature is 20 ℃，the most appropriate conditions are semi-light illumination． 4．The suitable hormone for Fritillaria przewalskii F．Przewalskii Maxim seedling rooting is MS+NAA0．5 mg／L+IBA0．2 mg／L+VBI mg／L，the most Suitable temperature is 1 5℃，the most appropriate light is the darkness． 5．The total alkaloid content of regenerate Fritillaria przewalskii E Przewalskii Maxim is higher than the total alkaloid content of natural Fritillaria przewalskii E Przewalskii Maxim． 6．The appropriate harvest time for regenerate Fritillaria pmewalskii F．Przewalskii Maxim is 60 days． 7．The suitable light for producting of Fritillaria pmewalSkii E Przewalskii Maxim’S total alkaloid is dark． 8．The suitable temper