藏药鲁堆多吉有效成分含量的测定及其总皂苷对大鼠睾丸间质细胞影响的研究民族医学专业论文.docxVIP

．!壅壁塑查兰竺奎竺兰些笙奎 {YIII11I■●LJ-JL18I 12I 111I■●J■■■oIII 8I 117II 藏党参总皂苷的含量高于潞党参总皂苷含量，而其总黄酮的含量 略低于潞党参总黄酮。 藏党参总皂苷低剂量促进睾丸间质细胞睾酮的分泌和cAMP的含 量，高剂量抑制睾丸间质细胞睾酮的分泌和cAMP的含量，推测藏党 参总皂苷通过cAMP影响睾丸间质细胞睾酮的分泌。 藏党参总皂苷能降低睾丸间质细胞MDA的含量和SOD的活力，同 时提高GSH的含量。推测藏党参总皂苷可能参与睾丸间质细胞自由基 的清除，减少自由基对睾丸间质细胞的损伤。 关键词藏党参，总皂苷，睾丸间质细胞 中央民族大学研究生毕业论文Abstract 中央民族大学研究生毕业论文 Abstract obj ective： In recent years．the number of Intertility has grown year by year．The reproductive health has become a focus problem in scientifie research‘．Soo wewe havenave totO findtln a drugdrug mat lS natural，non-toxic and effective for reproductive health． This study will provide scientific datas of Codonpsis thalictrifolia Wall．，a traditional Tibetan medicine，on male reproductive function．Determined the content of the total ponins andand flavonoidstlavonolds inm itIt firstly．StudiedtlrStly tudleo thethe effectsel／ects andand the mechanisms of total saponins of Codonpsis thalictrifolia Wall． on Leydig cells in vitro Secondly． Methods： Confirmed the best extract method of the total saponins of Codonpsis thalictrifolia Wall．by single factor experiment and then compared the activity components in Codonpsis thalictrifolia Wall．with Codonopsis(pilosula franch．夕Nnannf．． Isolated the leydig cells and incubated these cells in DMEM／F 1 2 medium following MTT colorimetfic assay．Measured the testosterone secretory volume with HPLC and cAMP content． Furthermore determined the content of MDA and GSH，and the vitality of SOD by ELISA． 中央民族人学研究生毕业论文Results： 中央民族人学研究生毕业论文 Results： When the extract concentration was up to 8 0％and extract times was 3，the yield of the total saponins was maximum． The total saponins of Codonpsis thalictrifolia Wall．and Codonopsis(pilosula franch．)Nnannf．were 3．02％and 2．48％ separately；and the total flavonoids of Codonpsis thalictrifolia Wall．and Codonopsis(pilosula franch．)Nnannf．were 0．0875％ and 0．092 1％separately． The isolated Leydig cells motility rate was confirmed by ’，_ trypan 191ue excluslon experiment． 1 rypan bl