苯并恶嗪酮类化合物对hepg2细胞生长的影响遗传学专业论文.docxVIP

北京协和医学院 北京协和医学院 中国医学科学院 硕士学位论文 Benzoxazine Compounds on The Growth of HepG2 CeHs Abstract Background and Aims Hyperlipidemia is a risk factor for cardiovascular disease and metabolic abnormalities，including atherosclerosis，obesity and metabolic syndrome，and SO on．Due to hi．gh rates of hypcrlipidemia,relevant cholesterol-lowering drugs become the study hotspot． Studies have confnmed that benzoxazine compounds Call adjust the concentration of plasma lipoprotein，reduce blood lipid，But there is rio further research on the mechanism of this compound．Benzoxazine compounds don’t have clinical application and related clinical research．These compounds may have great damages and side effects to liver cells．Thus limit their research and application． To study the effects of three kinds of benzoxazine compounds on the growth of HepG2 cells．And determine whether these compounds have to)(icities on hepatocytcs． Methods HepG2 Was incubated with different concentrations of benzoxazine(1u M，1 0uM，50uM，75uM，l OOuM)and without benzoxazine for 24h respectively．The proliferation rate of HepG2 Was determined by CCK-8 assay．The morphological changes of HepG2 were observed with inverted fluorescence microscope．With the Muse eell analyzer count． Results CCK8 assay indicated that the proliferation rate of HepG2 among different dose groups Was significantly reduced compared with control group，except the group whose concentration Was luM．HepG2 significantly inhibited the proliferation of HepG2 iIl a dose-dependent manner．After acridine orange staining，inverted fluorescence microscope showed the morpholo百cal changes of HepG2．n圮result of Muse cell analyzer shows that the count changes were more significant as the concentration increased． Condusion benzoxazine has inhibitory effect on cell proliferation of HepG2 cells，speculated that drugs may have toxic and side effects on the HepG2 cells． Keywords：Benzoxazine Compounds；HepG2 cells；toxic and side effects 2 万方数据 北京协和医学院 北京协和医学院