streptococcusthermophilustf96生长过程中胞内代谢特征与氨基酸利用模式研究生物化学与分子生物学专业论文.docxVIP

CONTENTSCONTENTS CONTENTS CONTENTS Abstract in Chinese．．． ．． ．． ．． ．． ．。 ．．． ． ．． ．， ．． ．。 ．． ．． ， ．．． ，】： Abstract in English．．． ． ． ．． ． ．．．．． ．． ． ． ． ． ． ． ．．III 1 Introduction． ． ． ． ． ． ．．．．． ． ．．． l 1．1 Background ofthe subject．． ．． ．． ．．．． ．． ．． ．． ．．． ． ．． ．． ． ． ．． ．1 1．2 The stress factors the grow oflactic acid bacteria ．2 1．2．1 Pressure oflactic acid bacteria physiological status ofintracellular acidity 3 1．2．2 Pressure of lactic acid bacteria physiological status of extracellular osmotic pressure 一4 1．3 Impact011lactic acidbactermphysiological status ofintracellularacidity． ．． ， ． ．． ．4 1．4 Impact lactic acid bacteria physiological status of extracellular osmotic pressure ．5 1．5 Domestic and foreign research dynamic ．． ．．．． ．． ． ．． ． ． ．． ．．．．．6 1．6 Purpose and meaning of study．．．． ．．． ．． ．． ．． ．． ．． ．．．． ．． ．． ． ． ． ．．． ． ．．．7 1．7 Contents of study ． ， ．． ． ．．． ．．．． ．．．．．．． ．． ．． ．． ．． ． ． ． ．．．8 1．8 Technical route of study ．．9 2 Materials and Methods． ． ， ．． ．．．．．．．． ．． ． ． ． ． ．1() 2．1 Experimental materials ．10 2．1．1 Experimental strain ．10 2．1．2 Composition ofmediu／n ．．10 2．1．3 Instruments and equipments 1l 2．1．4 Main reagents 12 2，2 Experimental me山ods ． ． ． ．．． ． ．． ． ．．14 2．2．1 The research ofthe growth ofStreptococcus thermophilus TF96 ．14 2．2．2 Measurement of intracellular pH and extracellular sodium laerate of Streptococcus thermophilus TF96．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．，．．．．．．．．．．．．．．．．．．．．．．14 2．2．3 Measurement of intracellular ATP and H+一ATPase of Streptococcus thermophilus TF96 ．． ． ．． ． ． ． ． ． ． ． ． ． ． ． l!； 2．2．4 Measurement of intracellular NADPH and NADH of Streptococcus thermophilus TF96 ．． ． ．．． ．． ． ．． ． ． ． ．．15 2．2．5 Measurement of iatracellular ammoaium ion of Streptococcus thermophilus