九节茶抗病毒作用的实验研究-中医临床基础专业论文.docxVIP

别为31．25，63．O，78．1 别为31．25，63．O，78．1 ug／ml，而鼻病毒的IC50为250 ug／ml。九节茶对以上四种病 毒的IC50分别为498．3，100，160．5和350．3，换算成原药材浓度(乘8．1)分别为 4．05mg／ml、0．81mg／ml、1．30mg／ml、2．83mg／ml。其治疗指数分别(TC50／IC50)为1．56， 7．8，20．25和2．31说明九节茶在体外对常见的引起呼吸道感染病毒具有较宽的抑制病 毒谱。 关键词：甲1型流感；九节茶；体内；体外； 抗病毒活性 Ⅱ The The Exper i menta I Study of Sarcandra glabra(Thunb．)Nakail on Influenza Vi rus FMl Strain SpecisI ty：The Basio soiences of OI inic of TraditionsI Chinese Bedioine with Western Medioine Author： Ma Mei Hsiang Tutor： Wang Xinhua ABSTRACT Influenza is a kind of infectious disease caused by influenza virus，which is one of orthomyxoviruses．Influenza virus has three kinds of serotype，A， B and C．There are several subtypes in every serotype．The categori zation based on the difference of Heamagglutinin(HA)and Neuraminidase(NA)．The influenza virus A has 13 HA subtypes and 9 NA ones．Among the total。HI～H3 and Nl～ N2 are correlated human influenza virse．The A virse，which exisets in the nature widely，can affect pourty，pigs，horses or any other animals，besides humanbeing．The B virus is found in human being only，and the C virus is epidemic in human and pigs．Jtillions of people were killed by influenza during the epidemic in the 20th century．Large manpower and material resources have been thrown into the study in attempt to find out effective ways to cure and prevent the infectious disease in every country． The herb roughhaired holly root has much effectiveness． such as heat—clearing；Promote the production of body fluid，promoting blood flow and detoxication．It is used in Guangdong herbal tea usually．Many Chinese doctors prescribe this herb for influenza and ShRS．While，there are no reports about anti—influenza virus of roughhaired holly root．The study aims to demonstrate the antiviral effect and mechanism of the herb． Literature review Review of the ancient 1iterature and up—dated data on the influenza virus were done．It is well recognized that the epidemics of influenza occurred at regular intervals．When the viral H a