碱性成纤维细胞生长因子调节小鼠MHC单倍体造血干细胞移植后急性移植物抗宿主病的实验分析-内科学(血液病)专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 10 PBS phosphate buffered solution 磷酸盐缓冲溶液 TNF-α tumor necrosis factor-α 肿瘤坏死因子-α TBI total body irradiation 全身照射 Study of bFGF preventing Acute GVHD in Murine Haploidentical Hematopoietic Stem Cell Transplantation Model Abstract Background and Objective： Hematopoietic stem cell transplantation (HSCT) is an effective method which can cure a variety of hematologic malignancies. However, For many patients we can not find a full-matched donor or only one locus mismatched related donors. In high risk patients , haploidentical donors are suitable alternatives.. Haploidentical stem cell transplantation has become an effective method for treatment of hematologic malignancies in recent years since we can easily get haploidentical donors from parents or child . Compared with full-matched transplantation, the GVHD (Graft-versus-host disease) is the main obstacle for the haploidentical hematopoietic stem cell transplantation (haplo-HSCT). The occurrence of a-GVHD after haplo-HSCT was reported from 50% to 80%, and the transplant-related mortality rate was 30% , which seriously affected the survival time and the quality of life .,And it is the main causes why haplo-HSCT can’t be widely apllied. In this study we want to find a new way to decrease the occurrence of a-GVHD after haplo-HSCT so as to decrease the complication and mortality rate of haplo-HSCT. The prevention methods for GVHD after Haplo-HSCT is mainly consist of removal of T cells in vitro, CD34 sorting, strengthing conditioning regimen and strengthing immune suppression taking. However, all the above methods have the disadvantages of high incidence of infection rate、recurrence rate、and transplant- related mortality rate. In recent years, cell therapy as a-GVHD prevention method in experimental study and clinical application got satisfactory effective, and the bone marrow-derived mesenchymal stem cells to prevent the effects of GVHD has been widely recognized. Prese