拮抗放线菌W7的筛选、鉴定及次生代谢产物的研究-生物化学与分子生物学专业论文.docxVIP

significant decrease. However, when the pH is 2, the diameter of the bacteriostatic circle was 15.7mm, and when the pH was 14, the diameter of the bacteriostatic circle changed into 12.6mm. What’s more, when kept at room temperature, the activity of metabolites was virtually unchanged. All the above indicated that the fermented broth of W7 was stable under the condition of acid and alkali, Ultraviolet lamplight, storage, hot, except strong acid/alkali. 6. The fermented broth was extracted using organic solvent. The active substance may be extracted by n-butanol, ethyl acetate and chloroform. Polarity had a great influence on the results. But the active metabolites could not be extracted by petroleum ether and benzene. The result of pH tomography showed that the Rf value of the active substances presented the degressive trend when the value of pH increased, which matched the atlas of acid antibiotic resistance. And that stated that w7 was acidic. While taking all the band ultraviolet scanning of the crude extract of the fermentation liquid, the absorbance peaks were at 295nm. And there was an oval inhibition zone under the TLC and biological developer method, which showed that it was probably identified as homogenous. Keywords: Antagonist actinomyces; Separation and screening; Optimization; Antibacterial active substances III 目录 摘要 I Abstract II HYPERLINK \l _bookmark0 第一部分 前言 1 HYPERLINK \l _bookmark1 1.1 农用抗生素的研究概况 1 HYPERLINK \l _bookmark2 1.1.1 杀菌抗生素 1 HYPERLINK \l _bookmark3 1.1.2 除草抗生素 2 HYPERLINK \l _bookmark4 1.1.3 杀虫抗生素 2 HYPERLINK \l _bookmark5 1.2 放线菌的多相分类 3 HYPERLINK \l _bookmark6 1.2.1 经典分类法 3 HYPERLINK \l _bookmark7 1.2.2 化学分类法 4 HYPERLINK \l _bookmark8 1.2.3 分子分类法 5 HYPERLINK \l _bookmark9 1.2.4 多相分类鉴定 6 HYPERLINK \l _bookmark10 1.3 抗生素的分离纯化 6 HYPERLINK \l _bookmark11 1.3.1 沉淀与结晶 6 HYPERLINK \l _bookmark12 1.3.2 溶媒萃取法 6 HYPERLINK \l _bookmark13 1.3.3 膜分离技术 6 HYPERLINK \l _bookmark14 1.3.4 色谱技术 7 HYPERLINK \l _bookmark15