拮抗解淀粉芽孢杆菌的筛选鉴定及其产胞外抗菌物质的初步分析-生物化学与分子生物学专业论文.docxVIP

曲阜师范大学 曲阜师范大学 2010 级硕士毕业论文 PAGE PAGE VI Abstract Plant disease causes huge economic loss to the global economy. The current approach to solve this problem is mainly using chemical fungicides and breeding new antibacterial varieties. However, chemical fungicides pollute the environment and threat the health of humans and animals. Antibacterial varieties need high economic cost and long-cycle cultivating in breeding. Due to the existing problems, the plant disease caused by pathogenic fungi can not be effectively controlled. Instead, using the bio-control mechanism of antagonistic bacteria to control plant disease is both efficient and environmental friendly. It has broad space. This study aimed at using twelve pathogens as indicators to get broad-spectrum antibacterial material-producing Bacillus amyloliquefaciens from more than three hundred strains of Bacillus of the laboratory and determining inhibitory spectrum of antimicrobial substances. Combined morphological analysis, physiological and biochemical characteristics, and 16S rDNA gene homology analysis, the antagonistic Bacillus obtained by screening was identified. Physiological and biochemical characteristics, optimization of fermentation conditions, prefractionation and purification of bio-control Bacillus were conducted. The main experimental methods and results are as follows: With nine pathogenic fungi and three pathogenic bacteria as indicators, the antagonistic Bacillus was screened using agar disk diffusion methods. In the end, antagonistic Bacillus of NCPSJ7 with broad antimicrobial spectrum was obtained. Morphological analysis, physiological and biochemical characteristics and 16S rDNA gene homology analysis were done for strain identification of antagonistic Bacillus of NCPSJ7. The results showed that the individual morphology, colony characteristics, physiological and biochemical characteristics of NCPSJ7 are similar with Bacillus amyloliquefaciens. By extracting genomic DNA, amplifying PCR, ana