结肠癌中HSP90β的表达意义以及与化疗敏感性的关系-外科学专业论文.docxVIP

中南大学硕士学位论文 中南大学硕士学位论文 中文摘要 (P0．05)，HSP90[3 mRNA在HCT-8／VCR细胞中的相对含量 (0．955：L-0．03)明显高于在HCT-8细胞中的相对含量 (0．835=1=0．029)(P0．05)，表明HSP90p的表达水平与HCT-8细胞对长 春新碱的耐药性有关系。 结论结肠癌组织中HSP90D的表达阳性率与水平均高于癌旁正 常组织，HSP90p的高表达可能参与了结肠癌的恶性转化。以长春新 碱处理结肠癌HCT-8细胞可以使该细胞对长春新碱的耐药性升高， HSP90p在细胞中的表达水平与细胞对长春新碱的耐药性有关系， HSP90[1的高表达可能参与了结肠癌细胞对化疗药物耐药性的产生。 关键词结肠癌，热休克蛋白90B，化学治疗，耐药 Ⅱ 中南大学硕士学位论文 中南大学硕士学位论文 英文摘要 Expression of HSV90p in human colon cancer and its relationship with chemotherapy sensitivity objective Colon cancer is one ofthe most common malignant tumor of digestive system．At present it is treated with combined therapy which the main is exakesis and the assistance is chemotherapy and radiotherapy．During chemotherapy，insensitivity oftumor cells to chemical drugs is the main cause leading to chemotherapeutic failure， which leads to reculTence and metastasis oftumor．Heat shock protein is a set ofproteins having general and significant biological function， which has a important role in cell growth，development and adjusting process as molecular chaperones．Heat shock protein 90 beta，which is ORe ofthe heat shock protein family，is one of factors leading to drug resistance oftumor cells．We studied the relationships between heat shock protein 90 beta and ooOulTence and development ofcolon cancer through observing expression difference ofheat shock protein 90 beta in normal colon tissue and colon CanCer tissue and its relation with tumor pathological classification and Duke’S stage；we initially illuminated dependability ofheat shock protein 90 beta and drug resistance ofcelon CanCA!lcells through contrasting expression difference ofheat shock protein 90 heta mRNA in colon c盈iiccr parent cells and drug-resistant cells． Methods 72 C翘SeS ofcolon cancer tissue samples and 30 cases of paracancer normal tissue samples Was colMcted in general surgery and hepatobiliaryenteric surgery ofXiang-Ya hospital during J